Rabbit anti-Human G3BP1 Polyclonal Antibody | anti-G3BP1 antibody

G3BP1 (Ab-232) Antibody

Cat. Num. AAA7131922

• Gene Names: G3BP1; G3BP; HDH-VIII
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were Purified by affinity-chromatography using epitope-specific peptide.
• Synonyms: G3BP1; Polyclonal Antibody; G3BP1 (Ab-232) Antibody; G3BP; GAP SH3-domain binding protein 1; anti-G3BP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were Purified by affinity-chromatography using epitope-specific peptide.
• Form/Format: Liquid; Supplied at 1.0mg/mL In Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% Sodium Azide and 50% Glycerol.

• Applicable Applications for anti-G3BP1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Peptide sequence around aa.230~234 (S-S-S-P-A) derived from Human G3BP-1.
• Immunogen Species: Human
• Conjugate: Non-conjugated
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western blot analysis of extracts from 293 cells using G3BP-1(Ab-232) Antibody and the same antibody preincubated with blocking peptide.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using G3BP-1(Ab-232) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

Product Categories/Family for anti-G3BP1 antibody

Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 5031703
• NCBI GeneID: 10146
• NCBI Accession #: NP_005745.1
• NCBI GenBank Nucleotide #: NM_005754.2
• UniProt Accession #: Q13283
• Molecular Weight: 52,164 Da
• NCBI Official Full Name: ras GTPase-activating protein-binding protein 1
• NCBI Official Synonym Full Names: GTPase activating protein (SH3 domain) binding protein 1
• NCBI Official Symbol: G3BP1
• NCBI Official Synonym Symbols: G3BP; HDH-VIII
• NCBI Protein Information: ras GTPase-activating protein-binding protein 1; G3BP-1; GAP binding protein; ATP-dependent DNA helicase VIII; GAP SH3 domain-binding protein 1; RasGAP-associated endoribonuclease G3BP; Ras-GTPase-activating protein SH3-domain-binding protein
• UniProt Protein Name: Ras GTPase-activating protein-binding protein 1
• Protein Family: Ras GTPase-activating protein-binding protein
• UniProt Gene Name: G3BP1
• UniProt Synonym Gene Names: G3BP; G3BP-1; hDH VIII
• UniProt Entry Name: G3BP1_HUMAN

NCBI Description

This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

G3BP-1: an hnRNA-binding protein and endoribonuclease that participates in the Ras signal transduction pathway. A regulated effector of stress granule (SG) assembly. SGs are involved in mRNA sorting in the storing of untranslated mRNAs. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA. Binds to the SH3 domain of Ras GTPase-activating protein (RasGAP) in proliferating cells but not in quiescent cells. Cytoplasmic in proliferating cells, can be recruited to the plasma membrane in exponentially growing cells. Cytosolic and partially nuclear in resting cells. Recruited to SGs upon either arsenite or high temperature treatment. RasGAP-dependent phosphorylation of S149 induces a conformational change that prevents self-association. Dephosphorylation after HRAS activation is required for stress granule assembly. S149 phosphorylation induces partial nuclear localization. A component of a TAU mRNP complex, Interacts with USP10, and may regulate it.

Protein type: EC 3.6.4.12; RNA-binding; Helicase; Adaptor/scaffold; EC 3.6.4.13

Chromosomal Location of Human Ortholog: 5q33.1

Cellular Component: focal adhesion; stress granule; cytoplasm; plasma membrane; cytosol; nucleus

Molecular Function: mRNA binding; ATP-dependent DNA helicase activity; protein binding; DNA binding; endonuclease activity; ATP-dependent RNA helicase activity; ATP binding

Biological Process: transport; metabolic process; Ras protein signal transduction; DNA duplex unwinding