Human Lipoprotein lipase ELISA Kit | LPL elisa kit

Human Lipoprotein lipase ELISA Kit

Cat. Num. AAA761180

• Gene Names: LPL; LIPD; HDLCQ11
• Reactivity: Human
• Synonyms: Lipoprotein lipase; Human Lipoprotein lipase ELISA Kit; LPL (Lipoprotein Lipase)/LIPD; LPL elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of LPL. No significant crossreactivity or interference between LPL and analogues was observed.
• Sequence Length: 475

• Samples: serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.781-50ng/ml
• Sensitivity: <0.469ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level LPL were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level LPL were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LPL elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- LPL antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- LPL antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the LPL amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of LPL can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4557727
• NCBI GeneID: 4023
• NCBI Accession #: NP_000228.1
• NCBI GenBank Nucleotide #: NM_000237.2
• Molecular Weight: 53,162 Da
• NCBI Official Full Name: lipoprotein lipase
• NCBI Official Synonym Full Names: lipoprotein lipase
• NCBI Official Symbol: LPL
• NCBI Official Synonym Symbols: LIPD; HDLCQ11
• NCBI Protein Information: lipoprotein lipase
• UniProt Protein Name: Lipoprotein lipase
• Protein Family: Lipoprotein
• UniProt Gene Name: LPL
• UniProt Synonym Gene Names: LIPD; LPL
• UniProt Entry Name: LIPL_HUMAN

NCBI Description

LPL encodes lipoprotein lipase, which is expressed in heart, muscle, and adipose tissue. LPL functions as a homodimer, and has the dual functions of triglyceride hydrolase and ligand/bridging factor for receptor-mediated lipoprotein uptake. Severe mutations that cause LPL deficiency result in type I hyperlipoproteinemia, while less extreme mutations in LPL are linked to many disorders of lipoprotein metabolism. [provided by RefSeq, Jul 2008]

Uniprot Description

LPL: The primary function of this lipase is the hydrolysis of triglycerides of circulating chylomicrons and very low density lipoproteins (VLDL). Binding to heparin sulfate proteogylcans at the cell surface is vital to the function. The apolipoprotein, APOC2, acts as a coactivator of LPL activity in the presence of lipids on the luminal surface of vascular endothelium. Defects in LPL are the cause of lipoprotein lipase deficiency (LPL deficiency); also known as familial chylomicronemia or hyperlipoproteinemia type I. LPL deficiency chylomicronemia is a recessive disorder usually manifesting in childhood. On a normal diet, patients often present with abdominal pain, hepatosplenomegaly, lipemia retinalis, eruptive xanthomata, and massive hypertriglyceridemia, sometimes complicated with acute pancreatitis. Belongs to the AB hydrolase superfamily. Lipase family.

Protein type: EC 3.1.1.34; Lipid Metabolism - glycerolipid; Phospholipase; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 8p22

Cellular Component: cell surface; chylomicron; extracellular matrix; extracellular region; extracellular space; plasma membrane

Molecular Function: apolipoprotein binding; heparin binding; lipoprotein lipase activity; phospholipase activity; protein binding; receptor binding; triacylglycerol lipase activity; triglyceride binding

Biological Process: fat-soluble vitamin metabolic process; fatty acid biosynthetic process; lipoprotein metabolic process; phospholipid metabolic process; phototransduction, visible light; response to cold; response to drug; response to glucose stimulus; retinoid metabolic process; triacylglycerol biosynthetic process; triacylglycerol catabolic process; triacylglycerol metabolic process; vitamin metabolic process

Disease: Hyperlipidemia, Familial Combined; Hyperlipoproteinemia, Type I