Western Blot (WB)
(Figure 1. Western blot analysis of PARP9 using anti-PARP9 antibody (MBS1753791).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat skeletal muscle tissue lysatesLane 2: mouse skeletal muscle tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-PARP9 antigen affinity purified polyclonal antibody (Catalog # MBS1753791) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for PARP9 approximately 130KD. The expected band size for PARP9 is at 96KD. )
Rabbit PARP9 Polyclonal Antibody | anti-PARP9 antibody
Anti-PARP9 Antibody
Gene Names
PARP9; BAL; BAL1; ARTD9; MGC:7868
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Purity
Immunogen affinity purified.
Synonyms
PARP9; Polyclonal Antibody; Anti-PARP9 Antibody; BAL; BAL1; Protein mono-ADP-ribosyltransferase PARP9; ADP-ribosyltransferase diphtheria toxin-like 9; ARTD9; B aggressive lymphoma protein; Poly [ADP-ribose] polymerase 9; PARP-9; poly(ADP-ribose) polymerase family member 9; anti-PARP9 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Rabbit IgG polyclonal antibody for PARP9 detection.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized. Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Applicable Applications for anti-PARP9 antibody
Western Blot (WB), Direct ELISA (EIA)
Application Notes
WB: 0.25-0.5ug/ml|Mouse, Rat|
Direct ELISA: 0.1-0.5ug/ml|Human|
Direct ELISA: 0.1-0.5ug/ml|Human|
Immunogen
E Coli-derived human PARP9 recombinant protein (Position: R117-E824).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Recommended Detection Systems
Recommended Detection Systems
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Western Blot (WB)
(Figure 1. Western blot analysis of PARP9 using anti-PARP9 antibody (MBS1753791).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat skeletal muscle tissue lysatesLane 2: mouse skeletal muscle tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-PARP9 antigen affinity purified polyclonal antibody (Catalog # MBS1753791) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for PARP9 approximately 130KD. The expected band size for PARP9 is at 96KD. )
Western Blot (WB)
(Figure 1. Western blot analysis of PARP9 using anti-PARP9 antibody (MBS1753791).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat skeletal muscle tissue lysatesLane 2: mouse skeletal muscle tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-PARP9 antigen affinity purified polyclonal antibody (Catalog # MBS1753791) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for PARP9 approximately 130KD. The expected band size for PARP9 is at 96KD. )
Related Product Information for anti-PARP9 antibody
Poly(ADP-ribosyl)ation is a post-translational modification of proteins mediated by one of the 17 members of the poly(ADP-ribose) polymerases (PARP). PARP-9 belongs to the subfamily of macroPARPs, associating one to three macro domains to the PARP domain. Overexpression of PARP-9 stimulates cell migration in vitro, suggesting a role for PARP-9 in the promotion of malignant B cell migration and dissemination in high risk DLBCL. PARP-9 is also likely a transcription coactivator, its overexpression in B lymphocytes, stimulated by IFNgamma, inducing the transcription of IFNgamma-controlled genes.
References
1. Aguiar, R. C. T., Takeyama, K., He, C., Kreinbrink, K., Shipp, M. A. B-aggressive lymphoma family proteins have unique domains that modulate transcription and exhibit poly(ADP-ribose) polymerase activity. J. Biol. Chem. 280: 33756-33765, 2005.
2. Aguiar, R. C. T., Yakushijin, Y., Kharbanda, S., Salgia, R., Fletcher, J. A., Shipp, M. A. BAL is a novel risk-related gene in diffuse large B-cell lymphomas that enhances cellular migration. Blood 96: 4328-4334, 2000.
3. Juszczynski, P., Kutok, J. L., Li, C., Mitra, J., Aguiar, R. C. T. BAL1 and BBAP are regulated by a gamma interferon-responsive bidirectional promoter and are overexpressed in diffuse large B-cell lymphomas with a prominent inflammatory infiltrate. Molec. Cell. Biol. 26: 5348-5359, 2006.
2. Aguiar, R. C. T., Yakushijin, Y., Kharbanda, S., Salgia, R., Fletcher, J. A., Shipp, M. A. BAL is a novel risk-related gene in diffuse large B-cell lymphomas that enhances cellular migration. Blood 96: 4328-4334, 2000.
3. Juszczynski, P., Kutok, J. L., Li, C., Mitra, J., Aguiar, R. C. T. BAL1 and BBAP are regulated by a gamma interferon-responsive bidirectional promoter and are overexpressed in diffuse large B-cell lymphomas with a prominent inflammatory infiltrate. Molec. Cell. Biol. 26: 5348-5359, 2006.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
80,291 Da
NCBI Official Full Name
poly
NCBI Official Synonym Full Names
poly (ADP-ribose) polymerase family, member 9
NCBI Official Symbol
PARP9
NCBI Official Synonym Symbols
BAL; BAL1; ARTD9; MGC:7868
NCBI Protein Information
poly [ADP-ribose] polymerase 9; poly [ADP-ribose] polymerase 9; PARP-9; b aggressive lymphoma protein; poly (ADP-ribose) polymerase 9; ADP-ribosyltransferase diphtheria toxin-like 9
UniProt Protein Name
Poly [ADP-ribose] polymerase 9
Protein Family
UniProt Gene Name
PARP9
UniProt Synonym Gene Names
BAL; BAL1; PARP-9; ARTD9
UniProt Entry Name
PARP9_HUMAN
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Product Notes
The PARP9 parp9 (Catalog #AAA1753791) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PARP9 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PARP9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Direct ELISA (EIA). WB: 0.25-0.5ug/ml|Mouse, Rat| Direct ELISA: 0.1-0.5ug/ml|Human|. Researchers should empirically determine the suitability of the PARP9 parp9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PARP9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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