A431+ EGF Lysate
A431+ EGF (5min) Lysate
Applications
Western Blot
Purity
Lysate
Synonyms
A431+ EGF; A431+ EGF (5min) Lysate; A431+ EGF lysate
Purity/Purification
Lysate
Form/Format
Supplied in electrophoresis sample buffer (62.5mM Tris pH 7.2, 2% SDS, 5% glycerol, 0.003% bromophenol blue, 0.9% b-mercaptoethanol).
Applicable Applications for A431+ EGF lysate
Western Blot (WB)
Application Notes
Suitable for use in SDS-PAGE/Western Blot.
Dilution: Western Blot: 20ul/well
Optimal dilutions to be determined by the researcher
Storage and Stability:
For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Dilution: Western Blot: 20ul/well
Optimal dilutions to be determined by the researcher
Storage and Stability:
For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Preparation and Storage
-20 degree C
Related Product Information for A431+ EGF lysate
A431 cells express approximately 10e6 epidermal growth factor (EGF) receptors at the cell surface. Upon stimulation with EGF, A431 cells exhibit a dramatic increase in phosphorylation of EGF receptors followed by activation of major cell signaling pathways, such as PKB/Akt and MAP kinase pathways. Downstream of these cell signaling pathways, a variety of cytoskeletal, cytoplasmic, and nuclear proteins become phosphorylated at different time points after EGF stimulation.
Confluent cultures of A431 cells were serum starved overnight. Cells were then either left untreated or treated with human EGF (100 ng/ml) for 30 minutes at 37 degree C.
Cells were lysed in 1% SDS, 1.0mM sodium ortho-vanadate, 10mM Tris (pH 7.4) buffer. Protein concentration was determined using the BCA method (Pierce) before diluting to final concentration and buffer.
Confluent cultures of A431 cells were serum starved overnight. Cells were then either left untreated or treated with human EGF (100 ng/ml) for 30 minutes at 37 degree C.
Cells were lysed in 1% SDS, 1.0mM sodium ortho-vanadate, 10mM Tris (pH 7.4) buffer. Protein concentration was determined using the BCA method (Pierce) before diluting to final concentration and buffer.
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Product Notes
The A431+ EGF (Catalog #AAA657264) is a Lysate and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's A431+ EGF can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in SDS-PAGE/Western Blot. Dilution: Western Blot: 20ul/well Optimal dilutions to be determined by the researcher Storage and Stability: For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Researchers should empirically determine the suitability of the A431+ EGF for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "A431+ EGF, Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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