Western Blot (WB)
(Western Blot analysis of A431, HeLa and Jurkat cells (20ug/lane) untreated or treated with pervanadate (1mM) for 30mins. Blots were probed with J9000-01.)
Jurkat Pervanadate Control Lysate
Jurkat Pervanadate Control Lysate
Applications
Western Blot
Purity
Lysate
Synonyms
Jurkat Pervanadate Control Lysate; Jurkat Pervanadate Control Lysate lysate
Purity/Purification
Lysate
Form/Format
Supplied in electrophoresis sample buffer (62.5mM Tris pH 7.2, 2% SDS, 5% glycerol, 0.003% bromophenol blue, 0.9% b-mercaptoethanol).
Applicable Applications for Jurkat Pervanadate Control Lysate lysate
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Dilution: Western Blot: 20ul/lane.
Confluent cultures of Jurkat cells were serum starved overnight. Cells were then either left untreated or treated with pervanadate (1mM) for 30 minutes at 37 degree C. Cells were lysed in 1% SDS, 1.0mM sodium ortho-vanadate, 10mM Tris (pH 7.4) buffer. Protein concentration was determined using the BCA method (Pierce) before diluting to final concentration and buffer.
Dilution: Western Blot: 20ul/lane.
Confluent cultures of Jurkat cells were serum starved overnight. Cells were then either left untreated or treated with pervanadate (1mM) for 30 minutes at 37 degree C. Cells were lysed in 1% SDS, 1.0mM sodium ortho-vanadate, 10mM Tris (pH 7.4) buffer. Protein concentration was determined using the BCA method (Pierce) before diluting to final concentration and buffer.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western Blot analysis of A431, HeLa and Jurkat cells (20ug/lane) untreated or treated with pervanadate (1mM) for 30mins. Blots were probed with J9000-01.)
Western Blot (WB)
(Western Blot analysis of A431, HeLa and Jurkat cells (20ug/lane) untreated or treated with pervanadate (1mM) for 30mins. Blots were probed with J9000-01.)
Related Product Information for Jurkat Pervanadate Control Lysate lysate
Pervanadate is a protein tyrosine phosphatase inhibitor that is commonly used to increase tyrosine phosphorylation in cells. When cells are treated with pervanadate for 30 minutes they undergo significant tyrosine phosphorylation, as shown by western blotting using anti-Phosphotyrosine. Confluent cultures of Jurkat cells were serum starved overnight. Cells were then either left untreated or treated with pervanadate (1 mM) for 30 minutes at 37 degree C. Cells were lysed in 1% SDS, 1.0mM sodium ortho-vanadate, 10mM Tris (pH 7.4) buffer. Protein concentration was determined using the BCA method (Pierce) before diluting to final concentration and buffer.
Similar Products
Product Notes
The Jurkat Pervanadate Control Lysate (Catalog #AAA657340) is a Lysate and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Jurkat Pervanadate Control Lysate can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 20ul/lane. Confluent cultures of Jurkat cells were serum starved overnight. Cells were then either left untreated or treated with pervanadate (1mM) for 30 minutes at 37 degree C. Cells were lysed in 1% SDS, 1.0mM sodium ortho-vanadate, 10mM Tris (pH 7.4) buffer. Protein concentration was determined using the BCA method (Pierce) before diluting to final concentration and buffer. Researchers should empirically determine the suitability of the Jurkat Pervanadate Control Lysate for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Jurkat Pervanadate Control Lysate, Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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