Lysophospholipase I Recombinant Protein | mLYPLA1 recombinant protein
Recombinant Mouse Lysophospholipase I
Gene Names
Lypla1; Pla1a
Purity
Greater than 90% as determined by SDS-PAGE.
Synonyms
Lysophospholipase I; Recombinant Mouse Lysophospholipase I; LYPLA1 Mouse; Lysophospholipase I Mouse Recombinant; Acyl-protein thioesterase 1; LYPLA1; APT-1; LPL1; LYSOPLA; mLYPLA1 recombinant protein
Host
E Coli
Purity/Purification
Greater than 90% as determined by SDS-PAGE.
Form/Format
The LYPLA1 protein solution (0.5mg/1ml) is formulated in 20mM Tris-HCl buffer (pH8.0)0.1M NaCl,1mM DTT and 10% glycerol.
Sterile Filtered clear solution.
Sterile Filtered clear solution.
Sequence
MGSSHHHHHH SSGLVPRGSH MCGNNMSAPM PAVVPAARKA TAAVIFLHGL GDTGHGWAEA FAGIKSPHIK YICPHAPVMP VTLNMNMAMP SWFDIVGLSP DSQEDESGIK QAAETVKALI DQEVKNGIPS NRIILGGFSQ GGALSLYTAL TTQQKLAGVT ALSCWLPLRA SFSQGPINSA NRDISVLQCH GDCDPLVPLM FGSLTVERLK ALINPANVTF KIYEGMMHSS CQQEMMDVKH FIDKLLPPID
Sequence Length
230
Preparation and Storage
Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Related Product Information for mLYPLA1 recombinant protein
Description: LYPLA1 produced in E Coli is a single, non-glycosylated polypeptide chain containing 250 amino acids (1-230a.a.) and having a molecular mass of 26.8kDa.LYPLA1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Introduction: LYPLA1 is lysophospholipase which performs on biological membranes to regulate the multifunctional lysophospholipids. LYPLA1 protein hydrolyzes fatty acids from S-acylated cysteine residues in proteins like trimeric G alpha proteins or HRAS and in addition has depalmitoylating activity.
Introduction: LYPLA1 is lysophospholipase which performs on biological membranes to regulate the multifunctional lysophospholipids. LYPLA1 protein hydrolyzes fatty acids from S-acylated cysteine residues in proteins like trimeric G alpha proteins or HRAS and in addition has depalmitoylating activity.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
23,367 Da
NCBI Official Full Name
acyl-protein thioesterase 1
NCBI Official Synonym Full Names
lysophospholipase 1
NCBI Official Symbol
Lypla1
NCBI Official Synonym Symbols
Pla1a
NCBI Protein Information
acyl-protein thioesterase 1; APT-1; LPL-I; lysoPLA I; lysophopholipase 1; lysophospholipase I; phospholipase 1a
UniProt Protein Name
Acyl-protein thioesterase 1
UniProt Gene Name
Lypla1
UniProt Synonym Gene Names
Apt1; Pla1a; APT-1; LPL-I; LysoPLA I
UniProt Entry Name
LYPA1_MOUSE
Uniprot Description
LYPLA1: Hydrolyzes fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS. Has depalmitoylating activity and also low lysophospholipase activity. Belongs to the AB hydrolase 2 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.1.2.-; Phospholipase; Lipid Metabolism - glycerophospholipid
Cellular Component: mitochondrion; cytoplasm; cytosol
Molecular Function: acyl-CoA hydrolase activity; hydrolase activity; palmitoyl-(protein) hydrolase activity; CoA hydrolase activity; lysophospholipase activity
Biological Process: negative regulation of Golgi to plasma membrane protein transport; lipid metabolic process; fatty acid metabolic process; protein depalmitoylation
