Hamster Lipoxin A4 ELISA Kit | LXA4 elisa kit

Hamster Lipoxin A4 (LXA4) ELISA Kit

Cat. Num. AAA281441

• Gene Names: FPR2; ALXR; HM63; FMLPX; FPR2A; FPRH1; FPRH2; FPRL1; LXA4R; FMLP-R-II
• Reactivity: Hamster
• Synonyms: Lipoxin A4; Hamster Lipoxin A4 (LXA4) ELISA Kit; LXA4 elisa kit

• Reactivity: Hamster
• Specificity: This assay has high sensitivity and excellent specificity for detection of Hamster (Mesocricetus; Cricetulus)LXA4. No significant cross-reactivity or interference between Hamster (Mesocricetus; Cricetulus)LXA4 and analogues was observed.
• Sequence Length: 351

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for LXA4 elisa kit

Lipoxins are a series of anti-inflammatory mediators. Lipoxins are short lived endogenously produced nonclassic eicosanoids whose appearance in inflammation signals the resolution of inflammation. They are abbreviated as LX, an acronym for lipoxygenase (LO) interaction products. At present two lipoxins have been identified; lipoxin A4 () and lipoxin B4 (LXB4).Lipoxins, as well as certain peptides, are high affinity ligands for the lipoxin A4 receptor (LXA4R), which was first identified based on sequence homology as the formyl peptide receptor like receptor (FPRL1). Lipoxin signaling through the LXA4R inhibits chemotaxis, transmigration, superoxide generation and NF-kappa B activation. Similarly to the leukotrienes, LXA4 will form the cysteinyl-lipoxins LXC4, LXD4 and LXE4. At subnanomolar concentrations, LXA4 and LXB4 inhibit leukotriene-stimulated interactions of human neutrophils and endothelial cells.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate LXA4 in samples. An antibody specific for LXA4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLXA4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LXA4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LXA4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 1916076
• NCBI GeneID: 2358
• Molecular Weight: 38,964 Da
• NCBI Official Full Name: lipoxin A4 receptor
• NCBI Official Synonym Full Names: formyl peptide receptor 2
• NCBI Official Symbol: FPR2
• NCBI Official Synonym Symbols: ALXR; HM63; FMLPX; FPR2A; FPRH1; FPRH2; FPRL1; LXA4R; FMLP-R-II
• NCBI Protein Information: N-formyl peptide receptor 2; RFP; FMLP-R-I; LXA4 receptor; FMLP-related receptor I; formyl peptide receptor-like 1; lipoxin A4 receptor (formyl peptide receptor related)
• UniProt Protein Name: N-formyl peptide receptor 2
• UniProt Gene Name: FPR2
• UniProt Synonym Gene Names: FPRH1; FPRL1; LXA4R; FMLP-R-I; LXA4 receptor
• UniProt Entry Name: FPR2_HUMAN

Uniprot Description

FPR2: Low affinity receptor for N-formyl-methionyl peptides, which are powerful neutrophils chemotactic factors. Binding of FMLP to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. The activation of LXA4R could result in an anti-inflammatory outcome counteracting the actions of proinflammatory signals such as LTB4 (leukotriene B4). Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Receptor, GPCR; Membrane protein, multi-pass; GPCR, family 1

Chromosomal Location of Human Ortholog: 19q13.3-q13.4

Cellular Component: plasma membrane; integral to membrane

Molecular Function: G-protein coupled receptor activity; N-formyl peptide receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; cell adhesion; inflammatory response; chemotaxis; cell motility