Goat Growth Differentiation Factor 2 ELISA Kit | GDF2 elisa kit
Goat Growth Differentiation Factor 2 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: GDF2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GDF2 antibody and an GDF2-HRP conjugate. The assay sample and buffer are incubated together with GDF2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GDF2 concentration since GDF2 from samples and GDF2-HRP conjugate compete for the anti-GDF2 antibody binding site. Since the number of sites is limited, as more sites are occupied by GDF2 from the sample, fewer sites are left to bind GDF2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GDF2 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Studies in rodents suggest that this protein plays a role in the adult liver and in differentiation of cholinergic central nervous system neurons. Mutations in this gene are associated with hereditary hemorrhagic telangiectasia. [provided by RefSeq, Jan 2014]
Uniprot Description
GDF2: Could be involved in bone formation. Belongs to the TGF-beta family.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 10q11.22
Cellular Component: extracellular space
Molecular Function: protein binding; growth factor activity; cytokine activity; transforming growth factor beta receptor binding
Biological Process: cellular iron ion homeostasis; positive regulation of transcription, DNA-dependent; activin receptor signaling pathway; positive regulation of interleukin-8 production; BMP signaling pathway; regulation of apoptosis; positive regulation of endothelial cell differentiation; angiogenesis; vasculogenesis; positive regulation of BMP signaling pathway; ossification; negative regulation of blood vessel endothelial cell migration; osteoblast differentiation; patterning of blood vessels; positive regulation of osteoblast differentiation; negative regulation of angiogenesis; positive regulation of angiogenesis; negative regulation of DNA replication; regulation of MAPKKK cascade; cartilage development; negative regulation of endothelial cell proliferation; blood vessel morphogenesis; positive regulation of endothelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of cell growth; cell development; growth
Disease: Telangiectasia, Hereditary Hemorrhagic, Type 5
