Leukotriene A4 Hydrolase Recombinant Protein | LTA4H recombinant protein

Recombinant Leukotriene A4 Hydrolase (LTA4H)

Cat. Num. AAA2031590

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Leukotriene A4 Hydrolase; Recombinant Leukotriene A4 Hydrolase (LTA4H); LTA4H recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; QCQAIH CRAILPCQDTPSVKLTYTAE VSVPKELVAL MSAIRDGEAP DPEDPSRKIY RFNQRVPIPCYLIALVVGAL ESRQIGPRTL VWSEKEQVEK SANEFSETES MLKIAEDLGGPYVWGQYDLL VLPPSFPYGG ME
• Sequence Length: 611

• Applicable Applications for LTA4H recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 19.1kDa
• Accurate Molecular Mass (KD): 19kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and S-tag
• Organism Species: Mus musculus (Mouse)
• Fragment: Gln135~Glu272
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 307131
• NCBI GeneID: 4048
• Molecular Weight: 66,852 Da
• NCBI Official Full Name: leukotriene A4 hydrolase
• NCBI Official Synonym Full Names: leukotriene A4 hydrolase
• NCBI Official Symbol: LTA4H
• NCBI Protein Information: leukotriene A-4 hydrolase
• UniProt Protein Name: Leukotriene A-4 hydrolase
• Protein Family: Leukotriene A-4 hydrolase
• UniProt Gene Name: LTA4H
• UniProt Synonym Gene Names: LTA4; LTA-4 hydrolase
• UniProt Entry Name: LKHA4_HUMAN

NCBI Description

The protein encoded by this gene is an enzyme that contains both hydrolase and aminopeptidase activities. The hydrolase activity is used in the final step of the biosynthesis of leukotriene B4, a proinflammatory mediator. The aminopeptidase activity has been shown to degrade proline-glycine-proline (PGP), a neutrophil chemoattractant and biomarker for chronic obstructive pulmonary disease (COPD). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]

Uniprot Description

LTA4H: Epoxide hydrolase that catalyzes the final step in the biosynthesis of the proinflammatory mediator leukotriene B4. Has also aminopeptidase activity. Belongs to the peptidase M1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid Metabolism - arachidonic acid; EC 3.3.2.6; Hydrolase

Chromosomal Location of Human Ortholog: 12q22

Cellular Component: nucleoplasm; cytoplasm; cytosol

Molecular Function: peptidase activity; leukotriene-A4 hydrolase activity; zinc ion binding; metallopeptidase activity; epoxide hydrolase activity; aminopeptidase activity

Biological Process: leukotriene biosynthetic process; peptide catabolic process; arachidonic acid metabolic process; leukotriene metabolic process; proteolysis; inflammatory response