LSM1 recombinant protein

LSM1 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121629

• Gene Names: LSM1; CASM; YJL124C
• Purity: >85% as determined by SDS-PAGE
• Synonyms: LSM1; LSM1 Protein; Human; Recombinant (His Tag); Human LSM1 Protein (His Tag); CASM Protein; YJL124C Protein; LSM1 homolog; mRNA degradation associated; LSM1 recombinant protein

• Host: E Coli
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 10% glycerol, 20mM Tris, 100mM NaCl, pH7.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Tyr133

• Species: Human
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the mature form of human LSM1 (O15116)(Met1-Tyr133) was expressed with a polyhistide tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for LSM1 recombinant protein

Background: LSM1 is an Sm-like protein. Sm-like proteins can be detected in a variety of organisms based on sequence homology with the Sm protein family. Sm-like proteins include the Sm sequence motif, which consists of two regions separated by a linker of variable length that folds as a loop. The Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing. LSM1 has a role in replication-dependent histone mRNA degradation and binds specifically to the 3''-terminal U-tract of U6 snRNA. LSM1 also facilitates RNA protein interactions and structural modifications which are required during ribosomal subunit assembly.

References

Shimizu Y, et al. (1997) Lineage- and differentiation stage-specific expression of LSM-1 (LPAP), a possible substrate for CD45, in human hematopoietic cells. Am J Hematol. 54(1):1=11.Graber MW, et al. (1997) CaSm: an Sm-like protein that contributes to the transformed state in cancer cells. Cancer Res. 57(14):2961-5.Sa©raphin B, et al. (1999) Sm and Sm-like proteins assemble in two related complexes of deep evolutionary origin. EMBO J. 18(12):3451-62.

NCBI and Uniprot Product Information

• NCBI GI #: 7657313
• NCBI GeneID: 27257
• NCBI Accession #: NP_055277.1
• NCBI GenBank Nucleotide #: NM_014462.2
• UniProt Accession #: O15116; B2R5E6
• Molecular Weight: 15,179 Da
• NCBI Official Full Name: U6 snRNA-associated Sm-like protein LSm1
• NCBI Official Synonym Full Names: LSM1, U6 small nuclear RNA associated
• NCBI Official Symbol: LSM1
• NCBI Official Synonym Symbols: CASM; YJL124C
• NCBI Protein Information: U6 snRNA-associated Sm-like protein LSm1; LSM1 homolog, U6 small nuclear RNA associated; cancer-associated Sm-like protein; small nuclear ribonuclear CaSm
• UniProt Protein Name: U6 snRNA-associated Sm-like protein LSm1
• Protein Family: Sm-like protein
• UniProt Gene Name: LSM1
• UniProt Synonym Gene Names: CASM
• UniProt Entry Name: LSM1_HUMAN

NCBI Description

This gene encodes a member of the LSm family of RNA-binding proteins. LSm proteins form stable heteromers that bind specifically to the 3'-terminal oligo(U) tract of U6 snRNA and may play a role in pre-mRNA splicing by mediating U4/U6 snRNP formation. Increased expression of this gene may play a role in cellular transformation and the progression of several malignancies including lung cancer, mesothelioma and breast cancer. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the short arm of chromosome 9. [provided by RefSeq, Nov 2011]

Uniprot Description

LSM1: Plays a role in replication-dependent histone mRNA degradation. Binds specifically to the 3'-terminal U-tract of U6 snRNA. Belongs to the snRNP Sm proteins family.

Protein type: RNA-binding

Chromosomal Location of Human Ortholog: 8p11.2

Cellular Component: cytoplasm; nucleus; cytosol

Molecular Function: protein binding; RNA cap binding

Biological Process: RNA splicing, via transesterification reactions; deadenylation-dependent decapping; RNA splicing; gene expression; mRNA processing; mRNA catabolic process, deadenylation-dependent decay