Rabbit anti-Human LSM1 Polyclonal Antibody | anti-LSM1 antibody

Anti-LSM1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8107243

• Gene Names: LSM1; CASM; YJL124C
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: LSM1; Polyclonal Antibody; Anti-LSM1 Antibody; Rabbit Polyclonal; LSM1 Antibody; Rabbit Pab; Antigen Affinity Purified; LSM1 homolog; mRNA degradation associated; Anti-CASM Antibody; Anti-YJL124C Antibody; anti-LSM1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human LSM1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-LSM1 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000; IHC-P: 1:2500-1:10,000; IP: 0.5-2uL/mg of lysate
• Immunogen: Recombinant Human LSM1 protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human LSM1 (rh LSM1). LSM1 specific IgG was purified by Human LSM1 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human LSM1 in human breast carcinoma with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of human LSM1 in human lung carcinoma with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of human LSM1 in human prostatic carcinoma with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).)

Immunoprecipitation (IP)

(LSM1 was immunoprecipitated using:Lane A:0.5 mg K562 Whole Cell Lysate2 uL anti-LSM1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-LSM1 rabbit polyclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 15 kDaObserved band size: 15 kDa)

Western Blot (WB)

(Anti-LSM1 rabbit polyclonal antibody at 1:500 dilutionLane A: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:15 kDaObserved band size:15 kDa)

Related Product Information for anti-LSM1 antibody

LSM1 is an Sm-like protein. Sm-like proteins can be detected in a variety of organisms based on sequence homology with the Sm protein family. Sm-like proteins include the Sm sequence motif, which consists of two regions separated by a linker of variable length that folds as a loop. The Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing. LSM1 has a role in replication-dependent histone mRNA degradation and binds specifically to the 3''-terminal U-tract of U6 snRNA. LSM1 also facilitates RNA protein interactions and structural modifications which are required during ribosomal subunit assembly.

References

Shimizu Y, et al. (1997) Lineage-and differentiation stage-specific expression of LSM-1 (LPAP), a possible substrate for CD45, in human hematopoietic cells. Am J Hematol. 54(1):1=11.Graber MW, et al. (1997) CaSm: an Sm-like protein that contributes to the transformed state in cancer cells. Cancer Res. 57(14):2961-5.Seraphin B, et al. (1999) Sm and Sm-like proteins assemble in two related complexes of deep evolutionary origin. EMBO J. 18(12):3451-62.

NCBI and Uniprot Product Information

• NCBI GI #: 7657313
• NCBI GeneID: 27257
• NCBI Accession #: NP_055277.1
• NCBI GenBank Nucleotide #: NM_014462.2
• UniProt Accession #: O15116; B2R5E6
• Molecular Weight: 15,179 Da
• NCBI Official Full Name: U6 snRNA-associated Sm-like protein LSm1
• NCBI Official Synonym Full Names: LSM1, U6 small nuclear RNA associated
• NCBI Official Symbol: LSM1
• NCBI Official Synonym Symbols: CASM; YJL124C
• NCBI Protein Information: U6 snRNA-associated Sm-like protein LSm1; LSM1 homolog, U6 small nuclear RNA associated; cancer-associated Sm-like protein; small nuclear ribonuclear CaSm
• UniProt Protein Name: U6 snRNA-associated Sm-like protein LSm1
• Protein Family: Sm-like protein
• UniProt Gene Name: LSM1
• UniProt Synonym Gene Names: CASM
• UniProt Entry Name: LSM1_HUMAN

NCBI Description

This gene encodes a member of the LSm family of RNA-binding proteins. LSm proteins form stable heteromers that bind specifically to the 3'-terminal oligo(U) tract of U6 snRNA and may play a role in pre-mRNA splicing by mediating U4/U6 snRNP formation. Increased expression of this gene may play a role in cellular transformation and the progression of several malignancies including lung cancer, mesothelioma and breast cancer. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the short arm of chromosome 9. [provided by RefSeq, Nov 2011]

Uniprot Description

LSM1: Plays a role in replication-dependent histone mRNA degradation. Binds specifically to the 3'-terminal U-tract of U6 snRNA. Belongs to the snRNP Sm proteins family.

Protein type: RNA-binding

Chromosomal Location of Human Ortholog: 8p11.2

Cellular Component: cytoplasm; nucleus; cytosol

Molecular Function: protein binding; RNA cap binding

Biological Process: RNA splicing, via transesterification reactions; deadenylation-dependent decapping; RNA splicing; gene expression; mRNA processing; mRNA catabolic process, deadenylation-dependent decay