Typical Testing Data/Standard Curve
Mouse Cbp/p300-interacting transactivator 2 (CITED2) ELISA Kit | CITED2 elisa kit
Mouse Cbp/p300-interacting transactivator 2 (CITED2) ELISA Kit
Typical Testing Data/Standard Curve
Typical Testing Data/Standard Curve
Principle of the Assay||CITED2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CITED2 antibody and an CITED2-HRP conjugate. The assay sample and buffer are incubated together with CITED2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CITED2 concentration since CITED2 from samples and CITED2-HRP conjugate compete for the anti-CITED2 antibody binding site. Since the number of sites is limited, as more sites are occupied by CITED2 from the sample, fewer sites are left to bind CITED2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CITED2 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene inhibits transactivation of HIF1A-induced genes by competing with binding of hypoxia-inducible factor 1-alpha to p300-CH1. Mutations in this gene are a cause of cardiac septal defects. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]
Uniprot Description
CITED2: Transcriptional coactivator of the p300/CBP-mediated trancription complex. Acts as a bridge, linking TFAP2 transcription factors and the p300/CBP transcriptional coactivator complex in order to stimulate TFAP2-mediated transcriptional activation. Positively regulates TGF-beta signaling through its association with the SMAD/p300/CBP-mediated transcriptional coactivator complex. Stimulates the peroxisome proliferator- activated receptors PPARA transcriptional activity. Enhances estrogen-dependent transactivation mediated by estrogen receptors. Acts also as a transcriptional corepressor; interferes with the binding of the transcription factors HIF1A or STAT2 and the p300/CBP transcriptional coactivator complex. Participates in sex determination and early gonad development by stimulating transcription activation of SRY. Plays a role in controlling left- right patterning during embryogenesis; potentiates transcriptional activation of NODAL-mediated gene transcription in the left lateral plate mesoderm (LPM). Plays an essential role in differentiation of the adrenal cortex from the adrenogonadal primordium (AGP); stimulates WT1-mediated transcription activation thereby up-regulating the nuclear hormone receptor NR5A1 promoter activity. Associates with chromatin to the PITX2 P1 promoter region. Defects in CITED2 are a cause of ventricular septal defect type 2 (VSD2). VSD2 is a common form of congenital cardiovascular anomaly that may occur alone or in combination with other cardiac malformations. It can affect any portion of the ventricular septum, resulting in abnormal communications between the two lower chambers of the heart. Classification is based on location of the communication, such as perimembranous, inlet, outlet (infundibular), central muscular, marginal muscular, or apical muscular defect. Large defects that go unrepaired may give rise to cardiac enlargement, congestive heart failure, pulmonary hypertension, Eisenmenger's syndrome, delayed fetal brain development, arrhythmias, and even sudden cardiac death. Defects in CITED2 are a cause of atrial septal defect type 8 (ASD8). ASD8 is a congenital heart malformation characterized by incomplete closure of the wall between the atria resulting in blood flow from the left to the right atria. Belongs to the CITED family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis; Apoptosis; Cell cycle regulation; Transcription, coactivator/corepressor; Nuclear receptor co-regulator
Chromosomal Location of Human Ortholog: 6q23.3
Cellular Component: nuclear chromatin; cytoplasm; nucleus
Molecular Function: histone acetyltransferase binding; protein binding; transcription coactivator activity; LBD domain binding; chromatin binding; transcription corepressor activity; transcription factor activity
Biological Process: spleen development; transcription, DNA-dependent; heart development; positive regulation of transcription, DNA-dependent; positive regulation of transforming growth factor beta receptor signaling pathway; positive regulation of cell cycle; negative regulation of transcription from RNA polymerase II promoter; liver development; sex determination; cell proliferation; response to estrogen stimulus; response to hypoxia; positive regulation of cell-cell adhesion; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; cell differentiation; determination of left/right symmetry; negative regulation of cell migration; negative regulation of apoptosis
Disease: Atrial Septal Defect 8; Ventricular Septal Defect 2
Research Articles on CITED2
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Product Notes
The Mouse CITED2 cited2 (Catalog #AAA7218751) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7218751 ELISA Kit recognizes Mouse CITED2. It is sometimes possible for the material contained within the vial of "Cbp/p300-interacting transactivator 2 (CITED2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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