LRPAP Recombinant Protein | Lrpap1 recombinant protein
LRPAP, Recombinant, Mouse (LDL Receptor-related Protein-Associated Protein 1)
Purity
Highly Purified95%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin 1EU/ug protein (LAL method)
Synonyms
LRPAP; Recombinant; Mouse (LDL Receptor-related Protein-Associated Protein 1); Lrpap1 recombinant protein
Host
E Coli
Purity/Purification
Highly Purified
95%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin 1EU/ug protein (LAL method)
95%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin 1EU/ug protein (LAL method)
Form/Format
Supplied as a lyophilized powder from PBS, pH 6.5. Reconstitute with sterile PBS to 0.1mg/ml.
Unit Definition
Measured by its ability to bind rcombinant mouse VLDLR with an estimated KD of <0.25nM.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Lrpap1 recombinant protein
LRPAP (LDL receptor-related protein-associated protein 1; also RAP) is a ubiquitously expressed 39kD molecular chaperone for LDL receptor family proteins. Mature mouse LRPAP is 332 amino acids (aa) in length and secreted into the ER/Golgi of the cell. It shares 77% and 97% aa sequence identity with human and rat LRPAP, respectively. LRPAP contains three approximately 100 aa alpha-helical domains (D1-D3). The D1 domain contains a low affinity binding site for LRP, and the associated D2 and D3 domains bind LRP with high affinity. Domains D2 and D3 interact with each other, while D1 is independent. The majority of LRPAP is localized in the endoplasmic reticulum and Golgi. LRPAP prevents the premature interaction of LRP, LRP2/megalin, and VLDLR with their coexpressed ligands, thereby promoting proper receptor folding and export from the ER. Protonation of conserved histidine residues within the D3 domain induces the separation of LRPAP and LRP in the relatively acidic Golgi. LRPAP, which contains a C-terminal HNEL motif, can then recycle to the ER. A minor amount of LRPAP remains associated with LRP and can modulate receptor activity on the cell surface. Exogenously applied LRPAP competitively inhibits LDL receptor family binding and uptake of activated alpha 2-macroglobulin, apoB100- or apoE-enriched LDL and VLDL particles, cholesteryl esters, and complexes of PAI-1 with either tPA or uPA.
DNA sequence encoding the mature mouse LRPAP (Gln 29-Leu 360; Accession # P55302) with a six residue histidine tag at the C-terminus, was expressed in E. coli.
DNA sequence encoding the mature mouse LRPAP (Gln 29-Leu 360; Accession # P55302) with a six residue histidine tag at the C-terminus, was expressed in E. coli.
Product Categories/Family for Lrpap1 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
39.9kD
NCBI Official Full Name
low density lipoprotein receptor-associated protein 1
UniProt Protein Name
Low density lipoprotein receptor-associated protein 1
Protein Family
UniProt Gene Name
Lrpap1
UniProt Synonym Gene Names
lrpap1
UniProt Entry Name
Q6ZY49_MOUSE
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Product Notes
The LRPAP lrpap1 (Catalog #AAA636642) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "LRPAP, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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