Chicken Low-density lipoprotein-receptor-related protein 6 ELISA Kit | LRP6 elisa kit

Chicken Low-density lipoprotein-receptor-related protein 6 ELISA Kit

Cat. Num. AAA736123

• Gene Names: LRP6; ADCAD2
• Reactivity: Chicken
• Synonyms: Low-density lipoprotein-receptor-related protein 6; Chicken Low-density lipoprotein-receptor-related protein 6 ELISA Kit; LRP6 elisa kit

• Reactivity: Chicken

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This LRP6 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken LRP6. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LRP6 elisa kit

Principle of the assay: LRP6 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-LRP6 antibody and an LRP6-HRP conjugate. The assay sample and buffer are incubated together with LRP6-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LRP6 concentration since LRP6 from samples and LRP6-HRP conjugate compete for the anti-LRP6 antibody binding site. Since the number of sites is limited, as more sites are occupied by LRP6 from the sample, fewer sites are left to bind LRP6-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LRP6 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for LRP6 elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 148727288
• NCBI GeneID: 4040
• NCBI Accession #: NP_002327.2
• NCBI GenBank Nucleotide #: NM_002336.2
• UniProt Accession #: O75581; Q17RZ2; Q59EX8; Q9UQH4
• Molecular Weight: 180,429 Da
• NCBI Official Full Name: low-density lipoprotein receptor-related protein 6
• NCBI Official Synonym Full Names: low density lipoprotein receptor-related protein 6
• NCBI Official Symbol: LRP6
• NCBI Official Synonym Symbols: ADCAD2
• NCBI Protein Information: low-density lipoprotein receptor-related protein 6; LRP-6
• UniProt Protein Name: Low-density lipoprotein receptor-related protein 6
• Protein Family: Low-density lipoprotein receptor-related protein
• UniProt Gene Name: LRP6
• UniProt Synonym Gene Names: LRP-6
• UniProt Entry Name: LRP6_HUMAN

NCBI Description

This gene encodes a member of the low density lipoprotein (LDL) receptor gene family. LDL receptors are transmembrane cell surface proteins involved in receptor-mediated endocytosis of lipoprotein and protein ligands. The protein encoded by this gene functions as a receptor or, with Frizzled, a co-receptor for Wnt and thereby transmits the canonical Wnt/beta-catenin signaling cascade. Through its interaction with the Wnt/beta-catenin signaling cascade this gene plays a role in the regulation of cell differentiation, proliferation, and migration and the development of many cancer types. This protein undergoes gamma-secretase dependent RIP- (regulated intramembrane proteolysis) processing but the precise locations of the cleavage sites have not been determined.[provided by RefSeq, Dec 2009]

Uniprot Description

LRP6: Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor- ligand complexes into ribosome-sized signalsomes. Cell-surface coreceptor of Wnt/beta-catenin signaling, which plays a pivotal role in bone formation. The Wnt-induced Fzd/LRP6 coreceptor complex recruits DVL1 polymers to the plasma membrane which, in turn, recruits the AXIN1/GSK3B-complex to the cell surface promoting the formation of signalsomes and inhibiting AXIN1/GSK3- mediated phosphorylation and destruction of beta-catenin. Required for posterior patterning of the epiblast during gastrulation. Homodimer; disulfide-linked. Forms phosphorylated oligomer aggregates on Wnt-signaling. Forms a WNT-signaling complex formed of a WNT protein, a FZD protein and LRP5 or LRP6. Interacts (via the extracellular domain) with WNT1; the interaction is enhanced by prior formation of the Wnt/Fzd complex. Interacts (via the beta-propeller regions 3 and 4) with WNT3A. Interacts (via the beta-propeller regions 1 and 2) with WNT9B. Interacts with FZD5; the interaction forms a coreceptor complex for Wnt signaling and is inhibited by DKK1 and C1orf187. Interacts (via beta propeller region) with DKK1; the interaction inhibits FZD5/LRP6 complex formation. Interacts with DKK2. Interacts with C1orf187/DRAXIN; the interaction inhibits Wnt signaling. Interacts (via the phosphorylated PPPSP motifs) with AXIN1; the interaction recruits the AXIN1/GSK3B complex to cell surface LRP6 signalsomes. Interacts with GRB10; the interaction prevents AXIN1 binding, thus negatively regulating the Wnt signaling pathway. Interacts (via the extracellular domain) with RSPO1; the interaction activates Wnt/beta-catenin signaling. Interacts (via the extracellular domain) with RSPO3 (via the cysteine rich domain); the interaction activates Wnt/beta-catenin signaling. Interacts (via the beta- propeller regions 1 and 2) with SOST; the interaction competes with DKK1 for binding for inhibiting beta-catenin signaling. Interacts with MESD; the interaction prevents the formation of LRP6 aggregates and targets LRP6 to the plasma membrane. Interacts (via the cytoplasmic domain) with CSNKIE; the interaction phosphorylates LRP6, binds AXIN1 and inhibits AXIN1/GSK3B-mediated phosphorylation of beta-catenin. Interacts with MACF1. Decreased levels on WNT3A stimulation. Widely co-expressed with LRP5 during embryogenesis and in adult tissues. Belongs to the LDLR family.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13.2

Cellular Component: Golgi apparatus; cell surface; endoplasmic reticulum; early endosome; integral to membrane; extracellular region; caveola; cell soma; early endosome membrane; plasma membrane; synapse; cytoplasmic vesicle; receptor complex

Molecular Function: low-density lipoprotein receptor activity; identical protein binding; Wnt-protein binding; Wnt receptor activity; protein binding; protein homodimerization activity; frizzled binding; kinase inhibitor activity; toxin transporter activity; apolipoprotein binding; receptor binding

Biological Process: embryonic forelimb morphogenesis; response to peptide hormone stimulus; radial glial cell differentiation in the forebrain; regulation of fat cell differentiation; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; cerebellum morphogenesis; palate development; thalamus development; Wnt receptor signaling pathway through beta-catenin; bone remodeling; embryonic pattern specification; embryonic hindlimb morphogenesis; Wnt receptor signaling pathway in forebrain neuroblast division; anterior/posterior pattern formation; synaptic transmission; elevation of cytosolic calcium ion concentration; cell-cell adhesion; negative regulation of protein amino acid phosphorylation; response to folic acid; convergent extension; positive regulation of mesenchymal cell proliferation; midbrain development; positive regulation of bone resorption; heart looping; neural crest cell differentiation; cell migration involved in gastrulation; embryonic limb morphogenesis; negative regulation of epithelial cell proliferation; external genitalia morphogenesis; Wnt receptor signaling pathway; neural crest formation; positive regulation of cell cycle; gastrulation with mouth forming second; negative regulation of fat cell differentiation; positive regulation of ossification; odontogenesis of dentine-containing teeth; formation of radial glial scaffolds; cerebral cortex cell migration; embryonic retina morphogenesis in camera-type eye; midbrain-hindbrain boundary development; neural tube closure; negative regulation of protein kinase activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; embryonic camera-type eye morphogenesis; embryonic digit morphogenesis; cerebral cortex development; regulation of ossification

Disease: Coronary Artery Disease, Autosomal Dominant 2