Human Low-density lipoprotein-receptor-related protein 1 ELISA Kit | LRP-1 elisa kit
Human Low-density lipoprotein-receptor-related protein 1 (LRP-1) ELISA Kit
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate LRP1 in samples. An antibody specific for LRP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLRP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LRP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LRP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the low-density lipoprotein receptor family of proteins. The encoded preproprotein is proteolytically processed by furin to generate 515 kDa and 85 kDa subunits that form the mature receptor (PMID: 8546712). This receptor is involved in several cellular processes, including intracellular signaling, lipid homeostasis, and clearance of apoptotic cells. In addition, the encoded protein is necessary for the alpha 2-macroglobulin-mediated clearance of secreted amyloid precursor protein and beta-amyloid, the main component of amyloid plaques found in Alzheimer patients. Expression of this gene decreases with age and has been found to be lower than controls in brain tissue from Alzheimer's disease patients. [provided by RefSeq, Oct 2015]
Uniprot Description
LRP1: a receptor involved in endocytosis, phagocytosis of apoptotic cells, and neural signaling. Modulates cellular events including APP metabolism, kinase-dependent intracellular signaling, neuronal calcium signaling and neurotransmission. Involved in the plasma clearance of chylomicron remnants and activated alpha 2-macroglobulin, as well as the local metabolism of complexes between plasminogen activators and their endogenous inhibitors. Plays a fundamental role in cellular signaling pathways in the brain. Mediates neural NMDA gating via a complex of LRP1, PSD-95 and NMDAR. A type I membrane protein. After cleavage, the intracellular domain (LRPICD) is detected both in the cytoplasm and in the nucleus. Most abundant in liver, brain and lung.
Protein type: Cell surface; Membrane protein, integral; Motility/polarity/chemotaxis; Receptor, misc.
Chromosomal Location of Human Ortholog: 12q13.3
Cellular Component: basolateral plasma membrane; cell soma; clathrin-coated pit; clathrin-coated vesicle; cytosol; dendrite; early endosome; focal adhesion; integral component of plasma membrane; lysosomal membrane; membrane; nucleolus; plasma membrane; plasma membrane protein complex; receptor complex
Molecular Function: alpha-2 macroglobulin receptor activity; apolipoprotein binding; apolipoprotein receptor activity; calcium ion binding; clathrin heavy chain binding; heparan sulfate proteoglycan binding; lipoprotein particle receptor binding; lipoprotein transporter activity; low-density lipoprotein receptor activity; protease binding; protein binding; protein complex binding; receptor activity; RNA binding; scavenger receptor activity
Biological Process: aging; apoptotic cell clearance; astrocyte activation during immune response; cell proliferation; cellular response to amyloid-beta; cerebral cortex development; lipid metabolic process; lipoprotein metabolic process; lipoprotein transport; negative regulation of neuron apoptosis; negative regulation of neuron projection development; negative regulation of platelet-derived growth factor receptor-beta signaling pathway; negative regulation of smooth muscle cell migration; negative regulation of Wnt signaling pathway; phagocytosis; positive regulation of amyloid-beta clearance; positive regulation of cell death; positive regulation of cholesterol efflux; positive regulation of endocytosis; positive regulation of lipid transport; positive regulation of lysosomal protein catabolic process; positive regulation of protein binding; positive regulation of protein catabolic process; positive regulation of protein transport; protein kinase C-activating G-protein coupled receptor signaling pathway; receptor internalization; receptor-mediated endocytosis; regulation of actin cytoskeleton organization; regulation of cholesterol transport; regulation of extracellular matrix disassembly; regulation of phospholipase A2 activity; regulation of protein metabolic process; retinoid metabolic process; transcytosis
Disease: Keratosis Pilaris Atrophicans
Research Articles on LRP-1
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Product Notes
The Human LRP-1 lrp1 (Catalog #AAA284769) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA284769 ELISA Kit recognizes Human LRP-1. It is sometimes possible for the material contained within the vial of "Low-density lipoprotein-receptor-related protein 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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