Typical Testing Data/Standard Curve (for reference only)
Rat lipoprotein lipase ELISA Kit | LPL elisa kit
Rat lipoprotein lipase, LPL ELISA Kit
Reactivity
Rat
Synonyms
lipoprotein lipase; Rat lipoprotein lipase; LPL ELISA Kit; HDLCQ11; LIPD; ; LPL elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse LPL. No significant cross-reactivity or interference between mouse LPL and analogues was observed.
Sequence Length
474
Samples
Serum, Plasma, Cell Culture Supernates, Tissue Homogenates
Assay Type
Quantitative Sandwich
Detection Range
0.625 ng/ml-40 ng/ml
Sensitivity
0.156 ng/ml.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for LPL elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LPL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LPL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LPL is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LPL bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
53,082 Da
NCBI Official Full Name
lipoprotein lipase
NCBI Official Synonym Full Names
lipoprotein lipase
NCBI Official Symbol
Lpl
NCBI Protein Information
lipoprotein lipase
UniProt Protein Name
Lipoprotein lipase
Protein Family
UniProt Gene Name
Lpl
UniProt Synonym Gene Names
LPL
UniProt Entry Name
LIPL_RAT
NCBI Description
lipase that hydrolyzes triglycerides; may be involved in the development of development of hypertriglyceridemia [RGD, Feb 2006]
Uniprot Description
Function: The primary function of this lipase is the hydrolysis of triglycerides of circulating chylomicrons and very low density lipoproteins (VLDL). Binding to heparin sulfate proteogylcans at the cell surface is vital to the function. The apolipoprotein, APOC2, acts as a coactivator of LPL activity in the presence of lipids on the luminal surface of vascular endothelium
Catalytic activity: Triacylglycerol + H2O = diacylglycerol + a carboxylate.
Research Articles on LPL
Similar Products
Product Notes
The Rat LPL lpl (Catalog #AAA703484) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA703484 ELISA Kit recognizes Rat LPL. It is sometimes possible for the material contained within the vial of "lipoprotein lipase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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