Rabbit anti-Human HIRIP3 Polyclonal Antibody | anti-HIRIP3 antibody

HIRIP3, ID (HIRIP3, HIRA-interacting protein 3) (Azide free) (HRP)

Cat. Num. AAA6306630

• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: HIRIP3; Polyclonal Antibody; ID (HIRIP3; HIRA-interacting protein 3) (Azide free) (HRP); HIRA-interacting protein 3; anti-HIRIP3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-HIRIP3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: HIRIP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-316 amino acids from the Central region of human HIRIP3.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(HIRIP3 Antibody (Center) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the HIRIP3 antibody detected the HIRIP3 protein (arrow).)

Related Product Information for anti-HIRIP3 antibody

The HIRA protein shares sequence similarity with Hir1p and Hir2p, the two corepressors of histone gene transcription characterized in the yeast, Saccharomyces cerevisiae. The structural features of the HIRA protein suggest that it may function as part of a multiprotein complex. Recently, several cDNAs encoding HIRA-interacting proteins, or HIRIPs, have been identified. In vitro, the HIRIP3 gene product binds HIRA, as well as H2B and H3 core histones, indicating that a complex containing HIRA-HIRIP3 could function in some aspects of chromatin and histone metabolism.

Product Categories/Family for anti-HIRIP3 antibody

Antibodies; Transcription Factors

References

Kumar, R.A., et al. PLoS ONE 4 (2), E4582 (2009): Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007). Assrir, N., et al. Biol. Chem. 388(4):391-398(2007). Olsen, J.V., et al. Cell 127(3):635-648(2006). Olsen, J.V., et al. Cell 127(3):635-648(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 344179118
• NCBI GeneID: 8479
• NCBI Accession #: NP_003600.2
• NCBI GenBank Nucleotide #: NM_003609
• UniProt Accession #: Q9BW71; O75707; O75708; H3BSR3
• Molecular Weight: 19,709 Da
• NCBI Official Full Name: Homo sapiens HIRA interacting protein 3 (HIRIP3), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: HIRA interacting protein 3
• NCBI Official Symbol: HIRIP3
• NCBI Protein Information: HIRA-interacting protein 3
• UniProt Protein Name: HIRA-interacting protein 3
• Protein Family: HIRA-interacting protein
• UniProt Gene Name: HIRIP3
• UniProt Entry Name: HIRP3_HUMAN

NCBI Description

The HIRA protein shares sequence similarity with Hir1p and Hir2p, the two corepressors of histone gene transcription characterized in the yeast, Saccharomyces cerevisiae. The structural features of the HIRA protein suggest that it may function as part of a multiprotein complex. Several cDNAs encoding HIRA-interacting proteins, or HIRIPs, have been identified. In vitro, the protein encoded by this gene binds HIRA, as well as H2B and H3 core histones, indicating that a complex containing HIRA-HIRIP3 could function in some aspects of chromatin and histone metabolism. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.[provided by RefSeq, Aug 2011]

Uniprot Description

HIRIP3: May play a role in chromatin function and histone metabolism via its interaction with HIRA and histones. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: nucleoplasm; nucleolus; nucleus

Molecular Function: protein binding

Biological Process: chromatin assembly or disassembly