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Typical Testing Data/Standard Curve (for reference only)

Human lipoprotein lipase ELISA Kit | LPL elisa kit

Human lipoprotein lipase, LPL ELISA Kit

Gene Names
LPL; LIPD; HDLCQ11
Reactivity
Human
Synonyms
lipoprotein lipase; Human lipoprotein lipase; LPL ELISA Kit; Human lipoprotein lipase (LPL) ELISA Kit; HDLCQ11; LIPD; ; LPL elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human LPL. No significant cross-reactivity or interference between human LPL and analogues was observed.
Sequence Length
475
Samples
Serum, plasma, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
31.25 pg/ml-2000 pg/ml
Sensitivity
<7.8 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for LPL elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LPL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LPL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LPL is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LPL bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
53,162 Da
NCBI Official Full Name
lipoprotein lipase
NCBI Official Synonym Full Names
lipoprotein lipase
NCBI Official Symbol
LPL
NCBI Official Synonym Symbols
LIPD; HDLCQ11
NCBI Protein Information
lipoprotein lipase
UniProt Protein Name
Lipoprotein lipase
Protein Family
UniProt Gene Name
LPL
UniProt Synonym Gene Names
LIPD; LPL
UniProt Entry Name
LIPL_HUMAN

NCBI Description

LPL encodes lipoprotein lipase, which is expressed in heart, muscle, and adipose tissue. LPL functions as a homodimer, and has the dual functions of triglyceride hydrolase and ligand/bridging factor for receptor-mediated lipoprotein uptake. Severe mutations that cause LPL deficiency result in type I hyperlipoproteinemia, while less extreme mutations in LPL are linked to many disorders of lipoprotein metabolism. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: The primary function of this lipase is the hydrolysis of triglycerides of circulating chylomicrons and very low density lipoproteins (VLDL). Binding to heparin sulfate proteogylcans at the cell surface is vital to the function. The apolipoprotein, APOC2, acts as a coactivator of LPL activity in the presence of lipids on the luminal surface of vascular endothelium

Catalytic activity: Triacylglycerol + H2O = diacylglycerol + a carboxylate.1 PublicationManual assertion based on experiment in:Ref.11

Research Articles on LPL

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Product Notes

The Human LPL lpl (Catalog #AAA701093) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA701093 ELISA Kit recognizes Human LPL. It is sometimes possible for the material contained within the vial of "lipoprotein lipase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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