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Typical Testing Data/Standard Curve

Rat Lysyl Oxidase ELISA Kit | LOX elisa kit

Rat Lysyl Oxidase (LOX) ELISA Kit

Reactivity
Rat
Synonyms
Lysyl Oxidase; Rat Lysyl Oxidase (LOX) ELISA Kit; Protein-Lysine 6-Oxidase; LOX elisa kit
Ordering
For Research Use Only!
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of Lysyl Oxidase (LOX).
No significant cross-reactivity or interference between Lysyl Oxidase (LOX) and analogues was observed.
Sequence Length
417
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma, Tissue homogenates and Other Biological Fluids
Detection Range
0.625-40ng/mL
Sensitivity
< 0.246ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Lysyl Oxidase (LOX) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Lysyl Oxidase (LOX) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve

Typical Testing Data/Standard Curve
Related Product Information for LOX elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lysyl Oxidase (LOX). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lysyl Oxidase (LOX). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lysyl Oxidase (LOX), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lysyl Oxidase (LOX) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for LOX elisa kit

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
46,944 Da
NCBI Official Full Name
lysyl oxidase
NCBI Official Synonym Full Names
lysyl oxidase
NCBI Official Symbol
LOX
NCBI Protein Information
protein-lysine 6-oxidase
UniProt Protein Name
Protein-lysine 6-oxidase
Protein Family
UniProt Gene Name
LOX
UniProt Entry Name
LYOX_HUMAN

NCBI Description

This gene encodes a member of the lysyl oxidase family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate a regulatory propeptide and the mature enzyme. The copper-dependent amine oxidase activity of this enzyme functions in the crosslinking of collagens and elastin, while the propeptide may play a role in tumor suppression. [provided by RefSeq, Nov 2015]

Uniprot Description

LOX: Responsible for the post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. In addition to cross-linking of extracellular matrix proteins, may have a direct role in tumor suppression. Belongs to the lysyl oxidase family.

Protein type: Secreted, signal peptide; Oxidoreductase; EC 1.4.3.13; Secreted

Chromosomal Location of Human Ortholog: 5q23.2

Cellular Component: collagen; extracellular region; extracellular space; nucleus; proteinaceous extracellular matrix

Molecular Function: copper ion binding; protein binding; protein-lysine 6-oxidase activity

Biological Process: collagen fibril organization; elastic fiber assembly; extracellular matrix organization and biogenesis; heart development; lung development; protein modification process; response to drug; response to steroid hormone stimulus; wound healing

Disease: Cutis Laxa, Autosomal Recessive, Type Ia

Research Articles on LOX

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Product Notes

The Rat LOX lox (Catalog #AAA2031966) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2031966 ELISA Kit recognizes Rat LOX. It is sometimes possible for the material contained within the vial of "Lysyl Oxidase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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