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Western Blot (WB) (SIA8C Antibody (C-term) western blot analysis in HL-60 cell line lysates (15ug/lane).This demonstrates the SIA8C antibody detected the SIA8C protein (arrow).)

Rabbit anti-Human SIA8C Polyclonal Antibody | anti-SIA8C antibody

SIA8C, CT (ST8SIA3, SIAT8C, Sia-alpha-2,3-Gal-beta-1,4-GlcNAc-R:alpha 2,8-sialyltransferase, Alpha-2,8-sialyltransferase 8C, Alpha-2,8-sialyltransferase III, ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 3, Sialyltransferase 8C, Sialytransfer

Gene Names
ST8SIA3; SIAT8C; ST8SiaIII
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
SIA8C; Polyclonal Antibody; CT (ST8SIA3; SIAT8C; Sia-alpha-2; 3-Gal-beta-1; 4-GlcNAc-R:alpha 2; 8-sialyltransferase; Alpha-2; 8-sialyltransferase 8C; 8-sialyltransferase III; ST8 alpha-N-acetyl-neuraminide alpha-2; 8-sialyltransferase 3; Sialyltransferase 8C; Sialytransfer; ST8SIA3; Sialytransferase St8Sia III; anti-SIA8C antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-SIA8C antibody
Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
SIA8C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 319-348 amino acids from the C-terminal region of human SIA8C.
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(SIA8C Antibody (C-term) western blot analysis in HL-60 cell line lysates (15ug/lane).This demonstrates the SIA8C antibody detected the SIA8C protein (arrow).)

Western Blot (WB) (SIA8C Antibody (C-term) western blot analysis in HL-60 cell line lysates (15ug/lane).This demonstrates the SIA8C antibody detected the SIA8C protein (arrow).)

Flow Cytometry (FC/FACS)

(SIA8C Antibody (C-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (SIA8C Antibody (C-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-SIA8C antibody
ST8SIA3 belongs to a family of sialyltransferases that form sialyl-alpha-2,8-sialyl-R linkages at the nonreducing termini of glycoconjugates (Lee et al., 1998 [PubMed 9826427]).
Product Categories/Family for anti-SIA8C antibody
References
Fellay, J., et al. PLoS Genet. 5 (12), E1000791 (2009): Kim, S.J., et al. Biochem. Biophys. Res. Commun. 344(4):1057-1064(2006). Angata, K., et al. J. Biol. Chem. 275(24):18594-18601(2000). Lee, Y.C., et al. Arch. Biochem. Biophys. 360(1):41-46(1998). Zeng, G., et al. Gene 187(1):131-134(1997).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
43,970 Da
NCBI Official Full Name
sia-alpha-2,3-Gal-beta-1,4-GlcNAc-R:alpha 2,8-sialyltransferase
NCBI Official Synonym Full Names
ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 3
NCBI Official Symbol
ST8SIA3
NCBI Official Synonym Symbols
SIAT8C; ST8SiaIII
NCBI Protein Information
sia-alpha-2,3-Gal-beta-1,4-GlcNAc-R:alpha 2,8-sialyltransferase
UniProt Protein Name
Sia-alpha-2,3-Gal-beta-1,4-GlcNAc-R:alpha 2,8-sialyltransferase
UniProt Gene Name
ST8SIA3
UniProt Synonym Gene Names
SIAT8C; SIAT8-C; ST8SiaIII
UniProt Entry Name
SIA8C_HUMAN

NCBI Description

ST8SIA3 belongs to a family of sialyltransferases that form sialyl-alpha-2,8-sialyl-R linkages at the nonreducing termini of glycoconjugates (Lee et al., 1998 [PubMed 9826427]).[supplied by OMIM, Mar 2008]

Uniprot Description

ST8SIA3: May transfer sialic acid through alpha-2,8-linkages to alpha-2,3-linked and alpha-2,8-linked sialic acid of N-linked oligosaccharides of glycoproteins and glycolipids. It can form polysialic acid in vitro directly on alpha-2,3-, alpha-2,6-, or alpha-2,8-linked sialic acid. Belongs to the glycosyltransferase 29 family.

Protein type: Membrane protein, integral; Transferase; EC 2.4.99.-; Glycan Metabolism - glycosphingolipid biosynthesis - ganglio series

Chromosomal Location of Human Ortholog: 18q21.31

Cellular Component: Golgi membrane; integral to Golgi membrane

Molecular Function: alpha-N-acetylneuraminate alpha-2,8-sialyltransferase activity; sialic acid binding

Biological Process: glycosphingolipid biosynthetic process; cellular protein metabolic process; dolichol-linked oligosaccharide biosynthetic process; glycoprotein metabolic process; protein amino acid glycosylation; protein amino acid N-linked glycosylation via asparagine; N-glycan processing; post-translational protein modification; oligosaccharide metabolic process; ganglioside biosynthetic process

Research Articles on SIA8C

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Product Notes

The SIA8C st8sia3 (Catalog #AAA6347515) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SIA8C, CT (ST8SIA3, SIAT8C, Sia-alpha-2,3-Gal-beta-1,4-GlcNAc-R:alpha 2,8-sialyltransferase, Alpha-2,8-sialyltransferase 8C, Alpha-2,8-sialyltransferase III, ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 3, Sialyltransferase 8C, Sialytransfer reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SIA8C can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Western Blot (WB). FC/FACS: 1:10-50 FLISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the SIA8C st8sia3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SIA8C, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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