Typical Testing Data/Standard Curve (for reference only)
Human Lysyl oxidase ELISA Kit | LOX elisa kit
Human Lysyl oxidase ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: LOX ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-LOX antibody and an LOX-HRP conjugate. The assay sample and buffer are incubated together with LOX-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LOX concentration since LOX from samples and LOX-HRP conjugate compete for the anti-LOX antibody binding site. Since the number of sites is limited, as more sites are occupied by LOX from the sample, fewer sites are left to bind LOX-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LOX concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is an extracellular copper enzyme that initiates the crosslinking of collagens and elastin. The enzyme catalyzes oxidative deamination of the epsilon-amino group in certain lysine and hydroxylysine residues of collagens and lysine residues of elastin. In addition to crosslinking extracellular matrix proteins, the encoded protein may have a role in tumor suppression. Defects in this gene are a cause of autosomal recessive cutis laxa type I (CL type I). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2010]
Uniprot Description
LOX: Responsible for the post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. In addition to cross-linking of extracellular matrix proteins, may have a direct role in tumor suppression. Belongs to the lysyl oxidase family.
Protein type: Oxidoreductase; Secreted; EC 1.4.3.13; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 5q23.2
Cellular Component: extracellular space; proteinaceous extracellular matrix; collagen; extracellular region; nucleus
Molecular Function: protein binding; copper ion binding; protein-lysine 6-oxidase activity
Biological Process: response to drug; blood vessel development; elastic fiber assembly; extracellular matrix organization and biogenesis; collagen fibril organization; wound healing; response to steroid hormone stimulus; protein modification process; lung development
Disease: Cutis Laxa, Autosomal Recessive, Type Ia
Research Articles on LOX
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Product Notes
The Human LOX lox (Catalog #AAA745192) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA745192 ELISA Kit recognizes Human LOX. It is sometimes possible for the material contained within the vial of "Lysyl oxidase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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