Human loricrin ELISA Kit | LOR elisa kit

Human Loricrin, LOR ELISA Kit

Cat. Num. AAA918132

• Reactivity: Human
• Synonyms: loricrin; Human Loricrin; LOR ELISA Kit; Human Loricrin (LOR) ELISA kit; MGC111513; ; LOR elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human LOR. No significant cross-reactivity or interference between human LOR and analogues was observed.
• Sequence Length: 312

• Samples: Serum, plasma, tissue homogenates, cell lysates
• Assay Type: Quantitative Sandwich
• Detection Range: 25 pg/ml-1600 pg/ml
• Sensitivity: < 6.25 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LOR elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LOR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LOR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LOR is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LOR bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 109255251
• NCBI GeneID: 4014
• NCBI Accession #: NP_000418.2
• NCBI GenBank Nucleotide #: NM_000427.2
• UniProt Accession #: P23490; Q5T869; Q5XKF8
• Molecular Weight: 25,761 Da
• NCBI Official Full Name: loricrin
• NCBI Official Synonym Full Names: loricrin
• NCBI Official Symbol: LOR
• NCBI Protein Information: loricrin
• UniProt Protein Name: Loricrin
• Protein Family: Loricrin
• UniProt Gene Name: LOR
• UniProt Synonym Gene Names: LRN
• UniProt Entry Name: LORI_HUMAN

NCBI Description

This gene encodes loricrin, a major protein component of the cornified cell envelope found in terminally differentiated epidermal cells. Mutations in this gene are associated with Vohwinkel's syndrome and progressive symmetric erythrokeratoderma, both inherited skin diseases. [provided by RefSeq, Jul 2008]

Uniprot Description

LOR: Major keratinocyte cell envelope protein. Defects in LOR are a cause of progressive symmetric erythrokeratodermia (PSEK). Erythrokeratodermas are a group of disorders characterized by widespread erythematous plaques, either stationary or migratory, associated with features that include palmoplantar keratoderma. PSEK is characterized by erythematous and hyperkeratotic plaques. Defects in LOR are the cause of Vohwinkel syndrome with ichthyosis (VSI); also known as loricrin keratoderma (LK) or mutilating keratoderma with ichthyosis. VSI is an ichthyotic variant of Vohwinkel syndrome (VS) characterized by progressive symmetric erythrokeratoderma or congenital ichthyosiform erythroderma born as a collodion baby. Common clinical features include hyperkeratosis of the palms and soles with digital constriction.

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: nucleoplasm; cytoplasm; cornified envelope

Molecular Function: protein binding, bridging; protein binding; structural constituent of cytoskeleton; structural molecule activity

Biological Process: keratinocyte differentiation; keratinization; peptide cross-linking

Disease: Vohwinkel Syndrome, Variant Form