Human Dihydrolipoyl Dehydrogenase (DLD) RTU ELISA Kit | DLD rtu elisa kit

Human Dihydrolipoyl Dehydrogenase (DLD) ELISA Kit

Cat. Num. AAA458868

• Gene Names: DLD; E3; LAD; DLDD; DLDH; GCSL; PHE3
• Reactivity: Human
• Synonyms: Dihydrolipoyl Dehydrogenase (DLD); Human Dihydrolipoyl Dehydrogenase (DLD) ELISA Kit; LDH-D; DLD; Probable D-lactate dehydrogenase; mitochondrial; DLD rtu elisa kit

• Reactivity: Human
• Sequence Length: 509

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates or Other Biological Fluids.
• Detection Range: 1.56-100U/L
• Sensitivity: 1.3U/L
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DLD rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to DLD. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to DLD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DLD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DLD in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 91199540
• NCBI GeneID: 1738
• NCBI Accession #: NP_000099.2
• NCBI GenBank Nucleotide #: NM_000108.4
• UniProt Accession #: P09622; Q14131; Q14167; Q59EV8; Q8WTS4; B2R5X0; B4DHG0; B4DT69
• Molecular Weight: 49,283 Da
• NCBI Official Full Name: dihydrolipoyl dehydrogenase, mitochondrial isoform 1
• NCBI Official Synonym Full Names: dihydrolipoamide dehydrogenase
• NCBI Official Symbol: DLD
• NCBI Official Synonym Symbols: E3; LAD; DLDD; DLDH; GCSL; PHE3
• NCBI Protein Information: dihydrolipoyl dehydrogenase, mitochondrial
• UniProt Protein Name: Dihydrolipoyl dehydrogenase, mitochondrial
• Protein Family: Delta-like protein
• UniProt Gene Name: DLD
• UniProt Synonym Gene Names: GCSL; LAD; PHE3

NCBI Description

This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In homodimeric form, the encoded protein functions as a dehydrogenase and is found in several multi-enzyme complexes that regulate energy metabolism. However, as a monomer, this protein can function as a protease. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

Lipoamide dehydrogenase is a component of the glycine cleavage system as well as an E3 component of three alpha-ketoacid dehydrogenase complexes (pyruvate-, alpha-ketoglutarate-, and branched-chain amino acid-dehydrogenase complex) (PubMed:15712224, PubMed:16442803, PubMed:16770810, PubMed:17404228, PubMed:20160912, PubMed:20385101). The 2-oxoglutarate dehydrogenase complex is mainly active in the mitochondrion (PubMed:29211711). A fraction of the 2-oxoglutarate dehydrogenase complex also localizes in the nucleus and is required for lysine succinylation of histones: associates with KAT2A on chromatin and provides succinyl-CoA to histone succinyltransferase KAT2A (PubMed:29211711). In monomeric form may have additional moonlighting function as serine protease (PubMed:17404228). Involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction ().