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Typical Testing Data/Standard Curve (for reference only)

Mouse LIM homeobox transcription factor 1-beta ELISA Kit | Lmx1b elisa kit

Mouse LIM homeobox transcription factor 1-beta ELISA Kit

Gene Names
Lmx1b; LMX1.1; LMX1.2
Reactivity
Mouse
Synonyms
LIM homeobox transcription factor 1-beta; Mouse LIM homeobox transcription factor 1-beta ELISA Kit; Lmx1b elisa kit
Ordering
For Research Use Only!
Reactivity
Mouse
Specificity
This assay recognizes recombinant and natural mouse LIM homeobox transcription factor 1-beta. No significant cross-reactivity or interference was observed.
Sequence Length
372
Assay Type
Sandwich
Detection Range
0.625-40 ng/ml
Sensitivity
0.328 ng/mL
Intra-Assay CV
<=8.3%
Inter-Assay CV
<=10.4%
Recovery
94%
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Lmx1b elisa kit
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to LIM homeobox transcription factor 1-beta. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for LIM homeobox transcription factor 1-beta and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain LIM homeobox transcription factor 1-beta, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of LIM homeobox transcription factor 1-beta in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
44,102 Da
NCBI Official Full Name
LIM homeobox transcription factor 1-beta
NCBI Official Synonym Full Names
LIM homeobox transcription factor 1 beta
NCBI Official Symbol
Lmx1b
NCBI Official Synonym Symbols
LMX1.1; LMX1.2
NCBI Protein Information
LIM homeobox transcription factor 1-beta
UniProt Protein Name
LIM homeobox transcription factor 1-beta
Protein Family
UniProt Gene Name
Lmx1b
UniProt Synonym Gene Names
LMX-1.2
UniProt Entry Name
LMX1B_MOUSE

Uniprot Description

LMX1B: Essential for the specification of dorsal limb fate at both the zeugopodal and autopodal levels. Defects in LMX1B are the cause of nail-patella syndrome (NPS); also known as onychoosteodysplasia. NPS is a disease that cause abnormal skeletal patterning and renal dysplasia. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Cell development/differentiation; Transcription factor

Cellular Component: nucleus

Molecular Function: DNA binding; metal ion binding; sequence-specific DNA binding; transcription factor activity; zinc ion binding

Biological Process: camera-type eye development; cell proliferation; central nervous system neuron development; cerebellum morphogenesis; collagen fibril organization; embryonic limb morphogenesis; limb morphogenesis; locomotory behavior; midbrain development; multicellular organismal development; neuron differentiation; neuron migration; organ growth; positive regulation of transcription from RNA polymerase II promoter; regulation of autophagy; regulation of gene expression; regulation of intracellular transport; regulation of synaptic plasticity; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; transcription, DNA-dependent

Research Articles on Lmx1b

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Product Notes

The Mouse Lmx1b lmx1b (Catalog #AAA288428) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA288428 ELISA Kit recognizes Mouse Lmx1b. It is sometimes possible for the material contained within the vial of "LIM homeobox transcription factor 1-beta, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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