Typical Testing Data/Standard Curve (for reference only)
Rat Bile salt sulfotransferase ELISA Kit | St2a1 elisa kit
Rat Bile salt sulfotransferase ELISA Kit
Gene Names
Sult2a1; STa; St2; Sth2; Smp-2; St2a1
Reactivity
Rat
Synonyms
Bile salt sulfotransferase; Rat Bile salt sulfotransferase ELISA Kit; St2a1 elisa kit
Reactivity
Rat
Specificity
This assay recognizes recombinant and natural rat Bile salt sulfotransferase. No significant cross-reactivity or interference was observed.
Sequence Length
285
Assay Type
Sandwich
Detection Range
78-5000 pg/mL
Sensitivity
0.091 ng/mL
Intra-Assay CV
<=4.4%
Inter-Assay CV
<=6.4%
Recovery
94%
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for St2a1 elisa kit
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bile salt sulfotransferase. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Bile salt sulfotransferase and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bile salt sulfotransferase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Bile salt sulfotransferase in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
33,191 Da
NCBI Official Full Name
bile salt sulfotransferase
NCBI Official Synonym Full Names
sulfotransferase family 2A, dehydroepiandrosterone (DHEA)-preferring, member 1
NCBI Official Symbol
Sult2a1
NCBI Official Synonym Symbols
STa; St2; Sth2; Smp-2; St2a1
NCBI Protein Information
bile salt sulfotransferase
UniProt Protein Name
Bile salt sulfotransferase
Protein Family
UniProt Gene Name
St2a1
UniProt Synonym Gene Names
ST; ST2A1
UniProt Entry Name
ST2A1_RAT
NCBI Description
catalyses the metabolic activation of potent carcinogenic polycyclic arylmethanols [RGD, Feb 2006]
Uniprot Description
SULT2A1: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfonation of steroids and bile acids in the liver and adrenal glands. Belongs to the sulfotransferase 1 family.
Protein type: Transferase; EC 2.8.2.14
Cellular Component: cytoplasm; cytosol
Molecular Function: 3'-phosphoadenosine 5'-phosphosulfate binding; alcohol sulfotransferase activity; bile-salt sulfotransferase activity; drug binding; sulfotransferase activity; transferase activity
Biological Process: aging; drug metabolic process; response to activity; response to insecticide; response to nutrient levels; steroid metabolic process; sulfation
Research Articles on St2a1
Similar Products
Product Notes
The Rat St2a1 st2a1 (Catalog #AAA288277) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA288277 ELISA Kit recognizes Rat St2a1. It is sometimes possible for the material contained within the vial of "Bile salt sulfotransferase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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