Mumps Virus Antigen
Mumps (Strain: Enders)
Applications
ELISA
Synonyms
Mumps Virus Antigen; Mumps (Strain: Enders); Mumps (Strain Enders) <10% Viral Protein; Mumps Virus Antigen antigen
Host
LLC-MK2 Cells
Form/Format
Liquid; 0.1M Glycine, pH 9.3 - 9.7
Applicable Applications for Mumps Virus Antigen antigen
ELISA
Application Notes
Each laboratory should determine an optimum working titier for use in its particular application.
Host Note
Grown in LLC-MK2 Cells
Product manufactured in the United States using only domestic animal materials procesed in U.S facilities.
Product manufactured in the United States using only domestic animal materials procesed in U.S facilities.
Protein Concentration
OD 260/280nm Assay:
Acceptable Range:0.6-1.0mg/ml
Testing Results: 0.87mg/ml
Acceptable Range:0.6-1.0mg/ml
Testing Results: 0.87mg/ml
Inactivation
Product inactivated by UV and verified by Infectivity Testing (cell Culture).
Acceptable Range: 0 CPE
Testing Results: Pass
Acceptable Range: 0 CPE
Testing Results: Pass
Activity
ELISA Assay: Reference Lot Number: M14138009
Testing Results: Midpoint Titer (test antigen): 601.51
Acceptable Range: -15% - +50% of Reference Antigen
% of reference: -0.69%
Testing Results Midpoint Titer (reference antigen): 605.68
Background ELISA Ratio: Acceptable <=2
Test Result: 0.9
Sensitivity/Specificity Negative Control: Acceptable to report
Test Result: Acceptable
Sensitivity/Specificity Medium Positive Control: Acceptable >=3
Test Result: 5.93
Sensitivity/Specificity High Positive Control: Acceptable >= 8
Test Result: 16.36
Testing Results: Midpoint Titer (test antigen): 601.51
Acceptable Range: -15% - +50% of Reference Antigen
% of reference: -0.69%
Testing Results Midpoint Titer (reference antigen): 605.68
Background ELISA Ratio: Acceptable <=2
Test Result: 0.9
Sensitivity/Specificity Negative Control: Acceptable to report
Test Result: Acceptable
Sensitivity/Specificity Medium Positive Control: Acceptable >=3
Test Result: 5.93
Sensitivity/Specificity High Positive Control: Acceptable >= 8
Test Result: 16.36
Viral Strain
Enders
Preparation and Storage
Store at <=-65°C.
Avoid multiple freeze/thaw cycles.
Shipping: Dry Ice.
Avoid multiple freeze/thaw cycles.
Shipping: Dry Ice.
Related Product Information for Mumps Virus Antigen antigen
Mumps Virus A
Introduction: Mumps Virus is a systemic viral disease of man. The most visibly apparent clinical symptom is demonstrated in two-thirds of infections, causing a characteristic swelling of salivary glands. Although other symptoms including sequela, meningioencephalitis, orchiditis leading to atrophy and some fetal wastage may be attributed to the disease. However, most cases are limited to nausea, vomiting, neck stiffness and headache. Control measures for mumps have been quite successful with the prevention of the disease through childhood vaccinations.
Serological identification of Mumps infection is the most common method of identification. Testing for the presence of Mumps antibody is a method of differentiating the disease from other parotitis-causing agents and to identify potential candidates for vaccination. The detection of IgM antibody appears to be the most sensitive method to indicate acute infection, with little cross-reactivity presented with related antigens. However, current IgG antibody test for Mumps virus appear to cross-react with parainfluenza virus antigens.
The Mumps antigen product MBS318648 is prepared from a glycine extraction of LLC-MK2 cells infected with the Mumps Virus strain Enders. The preparation is partially purified to reduce host cell components. The Mumps antigen is inactivated by ultraviolet light and is tested for infectivity prior to release. The purification process yields a Mumps antigen which has a high sensitivity and low background in the ELISA assay.
Introduction: Mumps Virus is a systemic viral disease of man. The most visibly apparent clinical symptom is demonstrated in two-thirds of infections, causing a characteristic swelling of salivary glands. Although other symptoms including sequela, meningioencephalitis, orchiditis leading to atrophy and some fetal wastage may be attributed to the disease. However, most cases are limited to nausea, vomiting, neck stiffness and headache. Control measures for mumps have been quite successful with the prevention of the disease through childhood vaccinations.
Serological identification of Mumps infection is the most common method of identification. Testing for the presence of Mumps antibody is a method of differentiating the disease from other parotitis-causing agents and to identify potential candidates for vaccination. The detection of IgM antibody appears to be the most sensitive method to indicate acute infection, with little cross-reactivity presented with related antigens. However, current IgG antibody test for Mumps virus appear to cross-react with parainfluenza virus antigens.
The Mumps antigen product MBS318648 is prepared from a glycine extraction of LLC-MK2 cells infected with the Mumps Virus strain Enders. The preparation is partially purified to reduce host cell components. The Mumps antigen is inactivated by ultraviolet light and is tested for infectivity prior to release. The purification process yields a Mumps antigen which has a high sensitivity and low background in the ELISA assay.
Product Categories/Family for Mumps Virus Antigen antigen
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Product Notes
The Mumps Virus Antigen (Catalog #AAA318648) is an Antigen produced from LLC-MK2 Cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Mumps Virus Antigen can be used in a range of immunoassay formats including, but not limited to, ELISA. Each laboratory should determine an optimum working titier for use in its particular application. Researchers should empirically determine the suitability of the Mumps Virus Antigen for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mumps Virus Antigen, Antigen" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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