Human IKAROS family zinc finger 3 (Aiolos) ELISA Kit | IKZF3 elisa kit

Human Zinc finger protein Aiolos, IKZF3 ELISA Kit

Cat. Num. AAA900945

• Gene Names: IKZF3; AIO; AIOLOS; ZNFN1A3
• Reactivity: Human
• Synonyms: IKAROS family zinc finger 3 (Aiolos); Human Zinc finger protein Aiolos; IKZF3 ELISA Kit; Human Zinc finger protein Aiolos (IKZF3) ELISA kit; AIO; AIOLOS; ZNFN1A3; aiolos; zinc finger protein; subfamily 1A; 3 (Aiolos) ; IKZF3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human IKZF3. No significant cross-reactivity or interference between human IKZF3 and analogues was observed.
• Sequence Length: 475

• Samples: Serum, plasma, tissue homogenates, cell lysates
• Assay Type: Quantitative Sandwich
• Detection Range: 25 pg/ml-1600 pg/ml
• Sensitivity: < 6.25 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IKZF3 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IKZF3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IKZF3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IKZF3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IKZF3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 383872639
• NCBI GeneID: 22806
• NCBI Accession #: NP_001244337.1
• NCBI GenBank Nucleotide #: NM_001257408.1
• UniProt Accession #: Q9UKT9; Q69BL6; Q69BL7; Q69BL8; Q69BL9; Q69BM0; Q69BM1; Q69BM2; Q69BM3; Q69BM5; Q8N574; B4DVV5
• Molecular Weight: 58,023 Da
• NCBI Official Full Name: zinc finger protein Aiolos isoform 7
• NCBI Official Synonym Full Names: IKAROS family zinc finger 3 (Aiolos)
• NCBI Official Symbol: IKZF3
• NCBI Official Synonym Symbols: AIO; AIOLOS; ZNFN1A3
• NCBI Protein Information: zinc finger protein Aiolos; zinc finger DNA binding protein Aiolos; zinc finger protein, subfamily 1A, 3 (Aiolos)
• UniProt Protein Name: Zinc finger protein Aiolos
• Protein Family: Zinc finger protein
• UniProt Gene Name: IKZF3
• UniProt Synonym Gene Names: ZNFN1A3
• UniProt Entry Name: IKZF3_HUMAN

NCBI Description

This gene encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This gene product is a transcription factor that is important in the regulation of B lymphocyte proliferation and differentiation. Both Ikaros and Aiolos can participate in chromatin remodeling. Regulation of gene expression in B lymphocytes by Aiolos is complex as it appears to require the sequential formation of Ikaros homodimers, Ikaros/Aiolos heterodimers, and Aiolos homodimers. Several alternative transcripts encoding different isoforms have been described, as well as some non-protein coding variants. [provided by RefSeq, Apr 2012]

Uniprot Description

Aiolos: a transcription factor of the ikaros C2H2-type zinc-finger protein family. Plays an important role in the regulation of lymphocyte differentiation. Deletions in Aiolos have been observed in a subset of pre-B-cell acute lymphoblastic leukemia (B-ALL) cases. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: C2H2-type zinc finger protein; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: cytoplasm; plasma membrane; nucleus

Molecular Function: protein binding; protein homodimerization activity; protein heterodimerization activity; sequence-specific DNA binding; metal ion binding; transcription factor activity

Biological Process: regulation of apoptosis; regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; B cell activation; regulation of lymphocyte differentiation; regulation of B cell differentiation; regulation of B cell proliferation; mesoderm development