Human DNA ligase 1 ELISA Kit | LIG1 elisa kit

Human DNA ligase 1 ELISA Kit

Cat. Num. AAA2883984

• Reactivity: Human
• Synonyms: DNA ligase 1; Human DNA ligase 1 ELISA Kit; DNA ligase I; Polydeoxyribonucleotide synthase [ATP] 1; LIG1; 6.5.1.1; LIG1 elisa kit

• Reactivity: Human
• Sequence Length: 919

• Samples: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates Or Other Biological Fluids.
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LIG1 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4557719
• NCBI GeneID: 3978
• NCBI Accession #: NP_000225.1
• NCBI GenBank Nucleotide #: NM_000234.2
• UniProt Accession #: P18858; Q2TB12; Q32P23; B2RAI8; B4DTU4
• Molecular Weight: 98,228 Da
• NCBI Official Full Name: DNA ligase 1 isoform 1
• NCBI Official Synonym Full Names: ligase I, DNA, ATP-dependent
• NCBI Official Symbol: LIG1
• NCBI Protein Information: DNA ligase 1
• UniProt Protein Name: DNA ligase 1
• Protein Family: DNA ligase
• UniProt Gene Name: LIG1
• UniProt Entry Name: DNLI1_HUMAN

NCBI Description

This gene encodes a member of the ATP-dependent DNA ligase protein family. The encoded protein functions in DNA replication, recombination, and the base excision repair process. Mutations in this gene that lead to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents. Disruption of this gene may also be associated with a variety of cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

LIG1: is an enzyme that functions in DNA replication and the base excision repair process. Mutations leading to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents.

Protein type: Ligase; EC 6.5.1.1; DNA repair, damage

Chromosomal Location of Human Ortholog: 19q13.2-q13.3

Cellular Component: Golgi apparatus; intracellular membrane-bound organelle; mitochondrion; nucleoplasm; nucleus

Molecular Function: ATP binding; DNA binding; DNA ligase (ATP) activity; DNA ligase activity; metal ion binding

Biological Process: anatomical structure morphogenesis; base-excision repair; cell division; DNA ligation; DNA ligation during DNA repair; DNA metabolic process; DNA repair; DNA strand elongation during DNA replication; double-strand break repair via nonhomologous end joining; mismatch repair; mitotic cell cycle; nucleotide-excision repair; nucleotide-excision repair, DNA gap filling; response to hydrogen peroxide; telomere maintenance; telomere maintenance via recombination; telomere maintenance via semi-conservative replication; transcription-coupled nucleotide-excision repair; V(D)J recombination