GPS2 Antibody | anti-GPS2 antibody

GPS2 Antibody

Cat. Num. AAA854099

• Gene Names: GPS2; AMF-1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: GPS2; GPS2 Antibody; G protein pathway suppressor 2; Protein GPS2; anti-GPS2 antibody

• Reactivity: Human, Mouse, Rat
• Specificity: GPS2 antibody detects endogenous levels of total GPS2 protein.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 327

• Applicable Applications for anti-GPS2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; ELISA: 1:40000
• Immunogen: The antiserum was produced against synthesized peptide derived from internal of human GPS2.
• Preparation and Storage: Store at-20 degree C for 1 year.

Western Blot (WB)

(Western blot analysis of extracts from RAW264.7 cells, using GPS2 antibody.)

Product Categories/Family for anti-GPS2 antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

Spain B.H., Mol. Cell. Biol. 16:6698-6706(1996). Bechtel S., BMC Genomics 8:399-399(2007). Zhang J., Mol. Cell 9:611-623(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 4758472
• NCBI GeneID: 2874
• NCBI Accession #: NP_004480.1
• NCBI GenBank Nucleotide #: NM_004489.4
• UniProt Accession #: Q13227; Q6FHM8; B4DXA1
• Molecular Weight: 33,907 Da
• NCBI Official Full Name: G protein pathway suppressor 2
• NCBI Official Synonym Full Names: G protein pathway suppressor 2
• NCBI Official Symbol: GPS2
• NCBI Official Synonym Symbols: AMF-1
• NCBI Protein Information: G protein pathway suppressor 2
• UniProt Protein Name: G protein pathway suppressor 2
• Protein Family: G protein pathway suppressor
• UniProt Gene Name: GPS2
• UniProt Synonym Gene Names: GPS-2

NCBI Description

This gene encodes a protein involved in G protein-mitogen-activated protein kinase (MAPK) signaling cascades. When overexpressed in mammalian cells, this gene could potently suppress a RAS- and MAPK-mediated signal and interfere with JNK activity, suggesting that the function of this gene may be signal repression. The encoded protein is an integral subunit of the NCOR1-HDAC3 (nuclear receptor corepressor 1-histone deacetylase 3) complex, and it was shown that the complex inhibits JNK activation through this subunit and thus could potentially provide an alternative mechanism for hormone-mediated antagonism of AP1 (activator protein 1) function. [provided by RefSeq, Jul 2008]

Uniprot Description

Key regulator of inflammation, lipid metabolism and mitochondrion homeostasis that acts by inhibiting the activity of the ubiquitin-conjugating enzyme UBE2N/Ubc13, thereby inhibiting 'Lys-63'-linked ubiquitination (). In the nucleus, can both acts as a corepressor and coactivator of transcription, depending on the context (PubMed:24943844). Acts as a transcription coactivator in adipocytes by promoting the recruitment of PPARG to promoters: acts by inhibiting the activity of the ubiquitin-conjugating enzyme UBE2N/Ubc13, leading to stabilization of KDM4A and subsequent histone H3 'Lys-9' (H3K9) demethylation (). Promotes cholesterol efflux by acting as a transcription coactivator (PubMed:19481530). Acts as a regulator of B-cell development by inhibiting UBE2N/Ubc13, thereby restricting the activation of Toll-like receptors (TLRs) and B-cell antigen receptors (BCRs) signaling pathways (). Acts as a key mediator of mitochondrial stress response: in response to mitochondrial depolarization, relocates from the mitochondria to the nucleus following desumoylation and specifically promotes expression of nuclear-encoded mitochondrial genes (PubMed:29499132). Promotes transcription of nuclear-encoded mitochondrial genes by inhibiting UBE2N/Ubc13 (PubMed:29499132). Can also act as a corepressor as part of the N-Cor repressor complex by repressing active PPARG (PubMed:19858209, PubMed:24943844). Plays an anti-inflammatory role in macrophages and is required for insulin sensitivity by acting as a corepressor (). Plays an anti-inflammatory role during the hepatic acute phase response by interacting with sumoylated NR1H2 and NR5A2 proteins, thereby preventing N-Cor corepressor complex dissociation (PubMed:20159957). In the cytosol, also plays a non-transcriptional role by regulating insulin signaling and pro-inflammatory pathways (). In the cytoplasm, acts as a negative regulator of inflammation by inhibiting the proinflammatory TNF-alpha pathway; acts by repressing UBE2N/Ubc13 activity (). In the cytoplasm of adipocytes, restricts the activation of insulin signaling via inhibition of UBE2N/Ubc13-mediated ubiquitination of AKT (). Able to suppress G-protein- and mitogen-activated protein kinase-mediated signal transduction (PubMed:8943324). Acts as a tumor-suppressor in liposarcoma (PubMed:27460081).