Typical Testing Data/Standard Curve (for reference only)
Canine Luteinizing Hormone Receptor ELISA Kit | LHR elisa kit
Canine Luteinizing Hormone Receptor ELISA Kit
Gene Names
LHCGR; HHG; LHR; LCGR; LGR2; ULG5; LHRHR; LSH-R; LH/CGR; LH/CG-R
Reactivity
Canine
Synonyms
Luteinizing Hormone Receptor; Canine Luteinizing Hormone Receptor ELISA Kit; LHR elisa kit
Reactivity
Canine
Specificity
This assay has high sensitivity and excellent specificity for detection of LHR. No significant cross-reactivity or interference between LHR and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between LHR and all the analogues, therefore, cross reaction may still exist in some cases.
Sequence Length
56
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
1.0 pg/mL
Reactivity Note
No significant cross-reactivity or interference between this antigen and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection, therefore, cross reaction may still exist in some cases.
Preparation and Storage
Store at 2-8 degree C.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for LHR elisa kit
Intended Uses: This LHR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine LHR. This ELISA kit for research use only!
Principle of the Assay: LHR ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-LHR antibody and an LHR-HRP conjugate. The assay sample and buffer are incubated together with LHR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LHR concentration since LHR from samples and LHR-HRP conjugate compete for the anti-LHR antibody binding site. Since the number of sites is limited, as more sites are occupied by LHR from the sample, fewer sites are left to bind LHR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LHR concentration in each sample is interpolated from this standard curve.
Principle of the Assay: LHR ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-LHR antibody and an LHR-HRP conjugate. The assay sample and buffer are incubated together with LHR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LHR concentration since LHR from samples and LHR-HRP conjugate compete for the anti-LHR antibody binding site. Since the number of sites is limited, as more sites are occupied by LHR from the sample, fewer sites are left to bind LHR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LHR concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
71,615 Da
NCBI Official Full Name
luteinizing hormone receptor, partial
NCBI Official Synonym Full Names
luteinizing hormone/choriogonadotropin receptor
NCBI Official Symbol
LHCGR
NCBI Official Synonym Symbols
HHG; LHR; LCGR; LGR2; ULG5; LHRHR; LSH-R; LH/CGR; LH/CG-R
NCBI Protein Information
lutropin-choriogonadotropic hormone receptor
UniProt Protein Name
Lutropin-choriogonadotropic hormone receptor
Protein Family
UniProt Gene Name
LHCGR
UniProt Synonym Gene Names
LCGR; LGR2; LHRHR; LH/CG-R; LHR; LSH-R
NCBI Description
This gene encodes the receptor for both luteinizing hormone and choriogonadotropin. This receptor belongs to the G-protein coupled receptor 1 family, and its activity is mediated by G proteins which activate adenylate cyclase. Mutations in this gene result in disorders of male secondary sexual character development, including familial male precocious puberty, also known as testotoxicosis, hypogonadotropic hypogonadism, Leydig cell adenoma with precocious puberty, and male pseudohermaphtoditism with Leydig cell hypoplasia. [provided by RefSeq, Jul 2008]
Uniprot Description
Receptor for lutropin-choriogonadotropic hormone (PubMed:11847099). The activity of this receptor is mediated by G proteins which activate adenylate cyclase (PubMed:11847099).
Research Articles on LHR
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Product Notes
The Canine LHR lhcgr (Catalog #AAA7262017) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7262017 ELISA Kit recognizes Canine LHR. It is sometimes possible for the material contained within the vial of "Luteinizing Hormone Receptor, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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