Human P2RY6 (P2Y purinoceptor 6) ELISA Kit | P2RY6 elisa kit

Human P2RY6 (P2Y purinoceptor 6) ELISA Kit

Cat. Num. AAA7612166

• Gene Names: P2RY6; P2Y6
• Reactivity: Human
• Synonyms: P2RY6 (P2Y purinoceptor 6); Human P2RY6 (P2Y purinoceptor 6) ELISA Kit; P2Y purinoceptor 6; P2Y6; PP2891; P2RY6; P2RY6 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of P2RY6. No significant cross-reactivity or interference between P2RY6 and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 31.25-2000pg/ml
• Sensitivity: 18.75pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for 6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for P2RY6 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 2495018
• NCBI GeneID: 5031
• UniProt Accession #: Q15077; Q15754
• Molecular Weight: 328
• NCBI Official Full Name: P2Y purinoceptor 6
• NCBI Official Synonym Full Names: pyrimidinergic receptor P2Y, G-protein coupled, 6
• NCBI Official Symbol: P2RY6
• NCBI Official Synonym Symbols: P2Y6
• NCBI Protein Information: P2Y purinoceptor 6; P2Y6 receptor; P2 purinoceptor; G-coupled nucleotide receptor
• UniProt Protein Name: P2Y purinoceptor 6
• Protein Family: P2Y purinoceptor
• UniProt Gene Name: P2RY6
• UniProt Synonym Gene Names: P2Y6
• UniProt Entry Name: P2RY6_HUMAN

NCBI Description

The product of this gene belongs to the family of P2 receptors, which is activated by extracellular nucleotides and subdivided into P2X ligand-gated ion channels and P2Y G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor, which is a G-protein coupled receptor, is responsive to UDP, partially responsive to UTP and ADP, and not responsive to ATP. It is proposed that this receptor mediates inflammatory responses. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Mar 2013]

Uniprot Description

P2Y6: Receptor for extracellular UDP > UTP > ATP. The activity of this receptor is mediated by G proteins which activate a phosphatidylinositol-calcium second messenger system. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, multi-pass; GPCR, family 1; Membrane protein, integral; Receptor, GPCR

Chromosomal Location of Human Ortholog: 11q13.5

Cellular Component: integral to plasma membrane; basolateral plasma membrane; apical plasma membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; UDP-activated nucleotide receptor activity; purinergic nucleotide receptor activity, G-protein coupled

Biological Process: transepithelial chloride transport; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of smooth muscle cell migration