LGMN recombinant protein

LGMN, 18-435aa, Mouse, His tag, Baculovirus

Cat. Num. AAA206321

• Gene Names: Lgmn; AEP; Prsc1; AI746452; AU022324
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: LGMN; 18-435aa; Mouse; His tag; Baculovirus; Legumain; AEP; AI746452; AU022324; Prsc1; LGMN recombinant protein

• Host: Baculovirus
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
• Concentration: 0.5 mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: VPVGVDDPED GGKHWVVIVA GSNGWYNYRH QADACHAYQI IHRNGIPDEQ IIVMMYDDIA NSEENPTPGV VINRPNGTDV YKGVLKDYTG EDVTPENFLA VLRGDAEAVK GKGSGKVLKS GPRDHVFIYF TDHGATGILV FPNDDLHVKD LNKTIRYMYE HKMYQKMVFY IEACESGSMM NHLPDDINVY ATTAANPKES SYACYYDEER GTYLGDWYSV NWMEDSDVED LTKETLHKQY HLVKSHTNTS HVMQYGNKSI STMKVMQFQG MKHRASSPIS LPPVTHLDLT PSPDVPLTIL KRKLLRTNDV KESQNLIGQI QQFLDARHVI EKSVHKIVSL LAGFGETAER HLSERTMLTA HDCYQEAVTH FRTHCFNWHS VTYEHALRYL YVLANLCEAP YPIDRIEMAM DKVCLSHYLE HHHHHH
• Sequence Length: 435

• Applicable Applications for LGMN recombinant protein: SDS-PAGE
• Antigen Species: Mouse
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for LGMN recombinant protein

LGMN, also known as legumain, is a lysosomal cysteine protease found in all mouse tissues, but was particularly abundant in kidney and placenta. It plays a pivotal role in the endosomal/lysosomal degradation system because the Legumain deficiency causes the accumulation of pro cathepsins B, H and L, another group of lysosomal cysteine proteases. Over expression of Legumain in tumors is significant for invasion/metastasis. Recombinant mouse LGMN, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for LGMN recombinant protein

Immunology

References

Shirahama-Noda K., et al. (2003) J. Biol. Chem. 278:33194-33199.; Liu C., et al. (2003) Cancer Res. 63:2957-2964.

NCBI and Uniprot Product Information

• NCBI GI #: 7242187
• NCBI GeneID: 19141
• NCBI Accession #: NP_035305
• NCBI GenBank Nucleotide #: NM_011175.3
• UniProt Accession #: O89017
• Molecular Weight: 48.6kDa (426aa) 40-57KDa (SDS-PAGE under reducing conditions)
• NCBI Official Full Name: legumain preproprotein
• NCBI Official Synonym Full Names: legumain
• NCBI Official Symbol: Lgmn
• NCBI Official Synonym Symbols: AEP; Prsc1; AI746452; AU022324
• NCBI Protein Information: legumain
• UniProt Protein Name: Legumain
• UniProt Gene Name: Lgmn
• UniProt Synonym Gene Names: Prsc1

NCBI Description

This gene encodes a member of the cysteine peptidase family C13 that plays an important role in the endosome/lysosomal degradation system. The encoded inactive preproprotein undergoes autocatalytic removal of the C-terminal inhibitory propeptide to generate the active endopeptidase that cleaves protein substrates on the C-terminal side of asparagine residues. Mice lacking the encoded protein exhibit defects in the lysosomal processing of proteins resulting in their accumulation in the lysosomes, and develop symptoms resembling hemophagocytic lymphohistiocytosis. [provided by RefSeq, Aug 2016]

Uniprot Description

Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system. Required for normal lysosomal protein degradation in renal proximal tubules. Required for normal degradation of internalized EGFR. Plays a role in the regulation of cell proliferation via its role in EGFR degradation.