Typical Testing Data/Standard Curve (for reference only)
(Human Galectin-7 PicoKine ELISA Kit standard curve )
Human Galectin-7 ELISA Kit | LGALS7 elisa kit
Human Galectin-7 PicoKine ELISA Kit
Gene Names
LGALS7; GAL7; LGALS7A
Reactivity
Human
Synonyms
Galectin-7; Human Galectin-7 PicoKine ELISA Kit; lectin; galactoside-binding; soluble; 7; GAL7; HKL14; Human keratinocyte lectin 14; Lectin galactoside binding soluble 7; LGALS7; LGALS7B; MGC151215; MGC151217; OTTMUSP00000025970; OTTMUSP00000038956; p53 induced gene 1 protein; PI7; PIG1; TP53I1; LGALS7 elisa kit
Reactivity
Human
Specificity
Natural and recombinant human Galectin-7
Sequence Length
136
Samples
Cell culture supernates, serum and plasma (heparin, EDTA)
Assay Type
Sandwich
Detection Range
156pg/ml-10,000pg/ml
Sensitivity
< 10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay)Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays)Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Typical Testing Data/Standard Curve (for reference only)
(Human Galectin-7 PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Human Galectin-7 PicoKine ELISA Kit standard curve )
Related Product Information for LGALS7 elisa kit
Principle of the assay: human Galectin-7 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Galectin-7 has been precoated onto 96-well plates. Standards(E.coli, S2-F136) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Galectin-7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Galectin-7 amount of sample captured in plate.
Background: Galectin-7, also known as GAL7, is a protein that in humans is encoded by the LGALS7 gene. This gene is mapped to 19q13.2. Galectins belong to a family of related beta-galactoside-binding lectins, also referred to as S-type or S-Lac lectins. Members of this family have been implicated in a variety of functions, including growth regulation, cell adhesion, migration, neoplastic transformation, and immune responses. Differential and in situ hybridizations indicate that this lectin is specifically expressed in keratinocytes. It has been found that expression of Galectin-7 was markedly upregulated in corneal epithelium after injury and that exogenous galectin-7 stimulated reepithelialization of corneal wounds.
Background: Galectin-7, also known as GAL7, is a protein that in humans is encoded by the LGALS7 gene. This gene is mapped to 19q13.2. Galectins belong to a family of related beta-galactoside-binding lectins, also referred to as S-type or S-Lac lectins. Members of this family have been implicated in a variety of functions, including growth regulation, cell adhesion, migration, neoplastic transformation, and immune responses. Differential and in situ hybridizations indicate that this lectin is specifically expressed in keratinocytes. It has been found that expression of Galectin-7 was markedly upregulated in corneal epithelium after injury and that exogenous galectin-7 stimulated reepithelialization of corneal wounds.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
15,075 Da
NCBI Official Full Name
Galectin-7
NCBI Official Synonym Full Names
lectin, galactoside-binding, soluble, 7
NCBI Official Symbol
LGALS7
NCBI Official Synonym Symbols
GAL7; LGALS7A
NCBI Protein Information
galectin-7; galectin 7
UniProt Protein Name
Galectin-7
Protein Family
UniProt Gene Name
LGALS7
UniProt Synonym Gene Names
PIG1; Gal-7
UniProt Entry Name
LEG7_HUMAN
NCBI Description
The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Differential and in situ hybridization studies indicate that this lectin is specifically expressed in keratinocytes and found mainly in stratified squamous epithelium. A duplicate copy of this gene (GeneID:653499) is found adjacent to, but on the opposite strand on chromosome 19. [provided by RefSeq, Jul 2008]
Uniprot Description
LGALS7B: Could be involved in cell-cell and/or cell-matrix interactions necessary for normal growth control. Pro-apoptotic protein that functions intracellularly upstream of JNK activation and cytochrome c release.
Protein type: Cell adhesion; Apoptosis
Chromosomal Location of Human Ortholog: 19q13.2
Cellular Component: extracellular space; cytoplasm; nucleus
Molecular Function: carbohydrate binding
Biological Process: heterophilic cell adhesion; apoptosis
Research Articles on LGALS7
Similar Products
Product Notes
The Human LGALS7 lgals7 (Catalog #AAA177204) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA177204 ELISA Kit recognizes Human LGALS7. It is sometimes possible for the material contained within the vial of "Galectin-7, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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