Human Galectin-3-binding protein ELISA Kit | LGALS3BP elisa kit

Human Galectin-3-binding protein ELISA Kit

Cat. Num. AAA2887742

• Gene Names: LGALS3BP; 90K; M2BP; gp90; CyCAP; BTBD17B; MAC-2-BP; TANGO10B
• Reactivity: Human
• Synonyms: Galectin-3-binding protein; Human Galectin-3-binding protein ELISA Kit; Basement membrane autoantigen p105; Lectin galactoside-binding soluble 3-binding protein; Mac-2-binding protein; MAC2BP; Mac-2 BP; Tumor-associated; LGALS3BP elisa kit

• Reactivity: Human
• Specificity: Recombinant and natural Human Galectin-3-binding protein
• Sequence Length: 585

• Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
• Detection Range: 0.78-50 ng/mL
• Sensitivity: < 0.34 ng/mL
• Intra-assay Precision: Intra-Assay CV: <=7.1%
• Inter-assay Precision: Inter-Assay CV: <=10.9%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LGALS3BP elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 5031863
• NCBI GeneID: 3959
• NCBI Accession #: NP_005558.1
• NCBI GenBank Nucleotide #: NM_005567.3
• UniProt Accession #: Q08380; Q7M4S0; Q9UCH8; Q9UCH9; Q9UCI0
• Molecular Weight: 65,331 Da
• NCBI Official Full Name: galectin-3-binding protein
• NCBI Official Synonym Full Names: lectin, galactoside-binding, soluble, 3 binding protein
• NCBI Official Symbol: LGALS3BP
• NCBI Official Synonym Symbols: 90K; M2BP; gp90; CyCAP; BTBD17B; MAC-2-BP; TANGO10B
• NCBI Protein Information: galectin-3-binding protein
• UniProt Protein Name: Galectin-3-binding protein
• Protein Family: Galectin-3-binding protein
• UniProt Gene Name: LGALS3BP
• UniProt Synonym Gene Names: M2BP; MAC2BP; Mac-2 BP
• UniProt Entry Name: LG3BP_HUMAN

NCBI Description

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. LGALS3BP has been found elevated in the serum of patients with cancer and in those infected by the human immunodeficiency virus (HIV). It appears to be implicated in immune response associated with natural killer (NK) and lymphokine-activated killer (LAK) cell cytotoxicity. Using fluorescence in situ hybridization the full length 90K cDNA has been localized to chromosome 17q25. The native protein binds specifically to a human macrophage-associated lectin known as Mac-2 and also binds galectin 1. [provided by RefSeq, Jul 2008]

Uniprot Description

LGALS3BP: Promotes intergrin-mediated cell adhesion. May stimulate host defense against viruses and tumor cells.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: extracellular matrix; extracellular space; membrane; proteinaceous extracellular matrix

Molecular Function: scavenger receptor activity

Biological Process: cell adhesion; cellular defense response; receptor-mediated endocytosis; signal transduction