Galectin-1 / LGALS1 Recombinant Protein | LGALS1 recombinant protein

Recombinant Mouse Galectin-1 / LGALS1 Protein

Cat. Num. AAA2547162

• Gene Names: Lgals1; L14; Gal-1; Galbp; L-14.5; Lect14; AA410090; galectin-1
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: Galectin-1 / LGALS1; Recombinant Mouse Galectin-1 / LGALS1 Protein; AA410090; Gal-1; Galbp; L-14.5; L14; Lect14; galectin-1; LGALS1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, 100mM beta-Lactose, pH 7.4
• Sequence Length: 135

• Application Notes: The recombinant mouse Lgals1 consists of 135 amino acids and migrates as an approximately 15 kDa band as predicted in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met 1
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

Related Product Information for LGALS1 recombinant protein

Background: Galectin-1 (Gal-1, GAL1), is a member of the galectins, a family of animal lectins ranging from Caenorhabditis elegans to humans, which is defined by their affinity for beta-galactosides and by significant sequence similarity in the carbohydrate-binding site. It is a homodimer with a subunit molecular mass of 14.5 kDa, which contains six cysteine residues per subunit. The cysteine residues should be in a free state in order to maintain a molecular structure that is capable of showing lectin activity. This endogenous lectin widely expressed at sites of inflammation and tumour growth, has been postulated as an attractive immunosuppressive agent to restore immune cell tolerance and homeostasis in autoimmune and inflammatory settings. On the other hand, galectin-1 contributes to different steps of tumour progression including cell adhesion, migration and tumour-immune escape, suggesting that blockade of galectin-1 might result in therapeutic benefits in cancer. Several potential glycoprotein ligands for galectin-1 have been identified, including lysosome-associated membrane glycoproteins and fibronectin, laminin, as well as T-cell glycoproteins CD43 and CD45. Evidence points to Gal-1 and its ligands as one of the master regulators of such immune responses as T-cell homeostasis and survival, T-cell immune disorders, inflammation and allergies as well as host-pathogen interactions.

Description: A DNA sequence encoding the mouse Lgals1 (P16045) (Met 1-Glu 135) was expressed and purified.

NCBI and Uniprot Product Information

• NCBI GI #: 6678682
• NCBI GeneID: 16852
• NCBI Accession #: NP_032521.1
• NCBI GenBank Nucleotide #: NM_008495.2
• UniProt Accession #: P16045; P05163; P11946; P17601; Q4FZH4; Q99M27; Q9D0X0
• Molecular Weight: 14,866 Da
• NCBI Official Full Name: galectin-1
• NCBI Official Synonym Full Names: lectin, galactose binding, soluble 1
• NCBI Official Symbol: Lgals1
• NCBI Official Synonym Symbols: L14; Gal-1; Galbp; L-14.5; Lect14; AA410090; galectin-1
• NCBI Protein Information: galectin-1
• UniProt Protein Name: Galectin-1
• UniProt Gene Name: Lgals1
• UniProt Synonym Gene Names: Gbp; Gal-1

Uniprot Description

galectin-1: May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Homodimer. Binds LGALS3BP. Interacts with CD2, CD3, CD4, CD7, CD43 and CD45. Interacts with laminin (via poly-N- acetyllactosamine). Expressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion. Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells.

Protein type: Cell adhesion; Extracellular matrix; Secreted

Chromosomal Location of Human Ortholog: 15 E1|15 37.7 cM

Cellular Component: cell surface; cytoplasm; cytosol; extracellular matrix; extracellular space; intracellular; nucleus

Molecular Function: carbohydrate binding; glycoprotein binding; lactose binding; laminin binding; protein binding; protein homodimerization activity; signal transducer activity

Biological Process: maintenance of protein localization; myoblast differentiation; plasma cell differentiation; positive regulation of erythrocyte aggregation; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of virion penetration into host cell; T cell costimulation