Typical Testing Data/Standard Curve (for reference only)
Human Pyruvate Dehydrogenase Phosphatase ELISA Kit | PDP elisa kit
Human Pyruvate Dehydrogenase Phosphatase ELISA Kit
Gene Names
Pdp; 153332_at; CG12151; DmelCG12151; pdp; PDP
Reactivity
Human
Synonyms
Pyruvate Dehydrogenase Phosphatase; Human Pyruvate Dehydrogenase Phosphatase ELISA Kit; PDP elisa kit
Reactivity
Human
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive or Sandwich
Detection Range
100-2500pg/mL
Sensitivity
1.0pg/mL
Intended Uses
This PDP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PDP. This ELISA kit for research use only, not for therapeutic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PDP elisa kit
Principle of the Assay: PDP ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PDP. Standards or samples are then added to the microtiter plate wells and PDP if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PDP present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for PDP are added to each well to "sandwich" the PDP immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PDP and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PDP concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
53,551 Da
NCBI Official Full Name
pyruvate dehydrogenase phosphatase, isoform B
NCBI Official Synonym Full Names
Pyruvate dehydrogenase phosphatase
NCBI Official Symbol
Pdp
NCBI Official Synonym Symbols
153332_at; CG12151; DmelCG12151; pdp; PDP
NCBI Protein Information
CG12151 gene product from transcript CG12151-RB; CG12151-PA; CG12151-PB; Pdp-PA; Pdp-PB; pyruvate dehydrogenase phosphatase
UniProt Protein Name
GM14286p
Protein Family
UniProt Gene Name
Pdp
UniProt Synonym Gene Names
CG12151; Dmel_CG12151
UniProt Entry Name
Q9W3Q1_DROME
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Product Notes
The Human PDP pdp (Catalog #AAA727807) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA727807 ELISA Kit recognizes Human PDP. It is sometimes possible for the material contained within the vial of "Pyruvate Dehydrogenase Phosphatase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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