Left/Right Determination Factor 1 (LEFTY1) Recombinant Protein | LEFTY1 recombinant protein

Recombinant Left/Right Determination Factor 1 (LEFTY1)

Cat. Num. AAA2011758

• Gene Names: LEFTY1; LEFTB; LEFTYB
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Left/Right Determination Factor 1 (LEFTY1); Recombinant Left/Right Determination Factor 1 (LEFTY1); LEFTY1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF- LVSV HESGWKAFDV TEAVNFWQQL SRPRQPLLLQ VSVQREHLGP LASGAHKLVR FASQGAPAGL GEPQLELHTL DLGDYGAQGD CDPEAPMTEG TRCCRQEMYI DLQGMKWAEN WVLEPPGFLA YECVGTCRQP PEALAFKWPF LGPRQCIASE TDSLPMIVSI KE
• Sequence Length: 366

• Applicable Applications for LEFTY1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Leu167~Glu332 (Accession # O75610) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 10337603
• NCBI GeneID: 10637
• NCBI Accession #: NP_066277.1
• NCBI GenBank Nucleotide #: NM_020997.3
• UniProt Accession #: O75610; Q53H67; Q5TE94
• Molecular Weight: 19.9kDa
• NCBI Official Full Name: left-right determination factor 1 preproprotein
• NCBI Official Synonym Full Names: left-right determination factor 1
• NCBI Official Symbol: LEFTY1
• NCBI Official Synonym Symbols: LEFTB; LEFTYB
• NCBI Protein Information: left-right determination factor 1; protein lefty-1; protein lefty-B; left-right determination factor B; left-right determination, factor B
• UniProt Protein Name: Left-right determination factor 1
• Protein Family: Left-right determination factor
• UniProt Gene Name: LEFTY1
• UniProt Synonym Gene Names: LEFTB; LEFTYB
• UniProt Entry Name: LFTY1_HUMAN

NCBI Description

This gene encodes a member of the TGF-beta family of proteins. A similar secreted protein in mouse plays a role in left-right asymmetry determination of organ systems during development. Alternative processing of this protein can yield three different products. This gene is closely linked to both a related family member and a related pseudogene. [provided by RefSeq, Jul 2008]

Uniprot Description

LEFTY1: Required for left-right axis determination as a regulator of LEFTY2 and NODAL. Belongs to the TGF-beta family.

Protein type: Motility/polarity/chemotaxis; Cytokine; Ligand, receptor tyrosine kinase; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1q42.1

Cellular Component: extracellular space

Molecular Function: growth factor activity; cytokine activity; transforming growth factor beta receptor binding

Biological Process: heart morphogenesis; regulation of apoptosis; regulation of MAPKKK cascade; transforming growth factor beta receptor signaling pathway; negative regulation of transcription from RNA polymerase II promoter; cell growth; determination of left/right symmetry; cell development