Testing Data
(Mouse pancreas in a vessel patency and drug delivery assay: (A) Ascinar Cells; (D) Ductal Cells; (I) Islet Cells. Functional vasculature labeled by injecting mouse with Lycopersicon Esculentum Lectin. Biotinylated Lycopersicon Esculentum Lectin is preincubated with streptavidin fluorophore (red) prior to injection. Drug delivery is evaluated by coadministering doxorubicin, an autofluorescent, small-molecule drug, with lectin (note green nuclei). DAPI counterstain (blue). Courtesy of Michael A Jacobetz, Cancer Research UK, Cambridge Research Institute, Cambridge, UK. Kenneth P. Olive, et al. Science 324, 1457 (2009); DOI: 10.1126/science.1171362.)
Lycopersicon esculentum Lectin
Lycopersicon esculentum Lectin (LEL, Tomato Lectin, TL) (Biotin)
Purity
Molecular Biology Grade
Synonyms
Lycopersicon esculentum Lectin; Lycopersicon esculentum Lectin (LEL; Tomato Lectin; TL) (Biotin); Lycopersicon esculentum Lectin lectin
Purity/Purification
Molecular Biology Grade
Form/Format
Supplied as a powder in 10mM HEPES, pH 7.5, 0.15M sodium chloride, 0.08% sodium azide, 0.1mM Ca++. Reconstitute with 0.5ml dH2O. Labeled with Biotin.
Concentration
~2mg/mL (after reconstitution) (varies by lot)
Application Notes
Suitable for use in ELISA. Other applications not tested.
Optimal dilutions to be determined by the researcher.
Optimal dilutions to be determined by the researcher.
Sugar Specificity
[GIcNac] 1-3, N-Acetylglucosamine
Preparation and Storage
Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Testing Data
(Mouse pancreas in a vessel patency and drug delivery assay: (A) Ascinar Cells; (D) Ductal Cells; (I) Islet Cells. Functional vasculature labeled by injecting mouse with Lycopersicon Esculentum Lectin. Biotinylated Lycopersicon Esculentum Lectin is preincubated with streptavidin fluorophore (red) prior to injection. Drug delivery is evaluated by coadministering doxorubicin, an autofluorescent, small-molecule drug, with lectin (note green nuclei). DAPI counterstain (blue). Courtesy of Michael A Jacobetz, Cancer Research UK, Cambridge Research Institute, Cambridge, UK. Kenneth P. Olive, et al. Science 324, 1457 (2009); DOI: 10.1126/science.1171362.)
Testing Data
(Mouse pancreas in a vessel patency and drug delivery assay: (A) Ascinar Cells; (D) Ductal Cells; (I) Islet Cells. Functional vasculature labeled by injecting mouse with Lycopersicon Esculentum Lectin. Biotinylated Lycopersicon Esculentum Lectin is preincubated with streptavidin fluorophore (red) prior to injection. Drug delivery is evaluated by coadministering doxorubicin, an autofluorescent, small-molecule drug, with lectin (note green nuclei). DAPI counterstain (blue). Courtesy of Michael A Jacobetz, Cancer Research UK, Cambridge Research Institute, Cambridge, UK. Kenneth P. Olive, et al. Science 324, 1457 (2009); DOI: 10.1126/science.1171362.)
Related Product Information for Lycopersicon esculentum Lectin lectin
Tomato lectin is a very stable glycoprotein containing about 50 percent arabinose and galactose. This lectin is composed of a single polypeptide of about 100,000D that may form aggregates in solution. Like other lectins that bind N-acetylglucosamine oligomers, tomato lectin prefers trimers and tetramers of this sugar. Tomato lectin, although sharing some specificities with potato, Datura lectin and wheat germ agglutinin, has been reported to be dissimilar in many respects. Tomato lectin binds well to such glycoproteins as glycophorin and Tamm-Horsfall glycoprotein. The tomato lectin complements our existing range of lectin reagents and should be a valuable tool in examining rodent tumor angiogenesis, tracing neovascular development in xenograft models and brain research. Tomato lectin (from Lycopersicon esculentum) is an effective marker of blood vessels and microglial cells in rodents. Conjugation of the lectin with biotin facilitates detection and visualization using intravascular perfusion methods or direct application to tissue sections. This biotinylated lectin conjugate is prepared from affinity-purified lectin and is optimally labeled with biotin. Essentially free of inactive lectin conjugate and containing no free biotin, this biotinylated lectin provides an ideal intermediate for examining glycoconjugates using the Biotin-Avidin System. First the biotin-labeled lectin is added, followed by an ABC Reagent, Avidin D conjugate, or streptavidin derivative. Another possible application is in the isolation of lymphokines and other products of mitogenic stimulation.
Product Categories/Family for Lycopersicon esculentum Lectin lectin
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Product Notes
The Lycopersicon esculentum Lectin (Catalog #AAA656828) is a Lectin and is intended for research purposes only. The product is available for immediate purchase. Suitable for use in ELISA. Other applications not tested. Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the Lycopersicon esculentum Lectin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Lycopersicon esculentum Lectin, Lectin" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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