Immunohistochemistry (IHC)

Lycopersicon esculentum Lectin

Lycopersicon esculentum Lectin (LEL, Tomato Lectin, TL) (FITC)

Applications

ELISA

Synonyms

Lycopersicon esculentum Lectin; Lycopersicon esculentum Lectin (LEL; Tomato Lectin; TL) (FITC); Lycopersicon esculentum Lectin lectin

Ordering
Cat. Num. AAA656566 For Research Use Only!

Form/Format

Supplied as a liquid in 10mM HEPES 0.15M sodium choride, pH 7.5, 0.08% sodium azide, 0.1 mM Ca++, 5 mg/mL B cyclodextrin. Labeled with Fluorescein isothiocyanate (FITC)

Concentration

2 mg/mL (varies by lot)

Applicable Applications for Lycopersicon esculentum Lectin lectin

Suitable for use in ELISA.Other applications not tested.

Application Notes

5-20 ug/mL

Optimal dilutions to be determined by the researcher.

Active Conjugate

99%

Inhibitin/Eluting Sugar

Chitin Hydrosylate

Excitation

495 nm

Emission

515 nm

F/P (molar)

2.1

Inhibiting/Eluting Sugar

Chitin Hydrolysate

Preparation and Storage

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunohistochemistry (IHC)

(Immunohistochemistry using L7785-25C; frozen section of colon viewed under fluorescein filter: Lycopersicon esculentum lectin nd glandular epithelium.)

Related Product Information for Lycopersicon esculentum Lectin lectin

Tomato lectin is a very stable glycoprotein containing about 50 percent arabinose and galactose. This lectin is composed of a single polypeptide of about 100,000D that may form aggregates in solution. Like other lectins that bind N-acetylglucosamine oligomers, tomato lectin prefers trimers and tetramers of this sugar. Tomato lectin, although sharing some specificities with potato, Datura lectin and wheat germ agglutinin, has been reported to be dissimilar in many respects. Tomato lectin binds well to such glycoproteins as glycophorin and Tamm-Horsfall glycoprotein. Tomato lectin (from Lycopersicon esculentum) is an effective marker of blood vessels and microglial cells in rodents. Conjugation of the lectin with a fluorophore facilitates fast, one-step detection and visualization using intravascular perfusion methods or direct application to tissue sections. The tomato lectin complements our existing range of lectin reagents and should be a valuable tool in examining rodent tumor angiogenesis, tracing neovascular development in xenograft models and brain research. Fluorescein labeled Lycopersicon Esculentum (Tomato) Lectin is produced by using the highest quality fluorescein isothiocyanate, our affinity-purified lectin, and special conjugation procedures. Fluorescein labeled Lycopersicon Esculentum (Tomato) Lectin has an appropriate number of fluorochromes bound which provide the maximum fluorescence and optimum staining characteristics for this particular lectin. This lectin is supplied essentially free of unconjugated fluorochromes and inactive lectin. Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions perserved with sodium azide.

Product Categories/Family for Lycopersicon esculentum Lectin lectin

Lectins; Lectin Lycopersicon esculentum

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Product Notes

The Lycopersicon esculentum Lectin (Catalog #AAA656566) is a Lectin and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Lycopersicon esculentum Lectin can be used in a range of immunoassay formats including, but not limited to, Suitable for use in ELISA.Other applications not tested. 5-20 ug/mL Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the Lycopersicon esculentum Lectin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Lycopersicon esculentum Lectin, Lectin" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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Lycopersicon esculentum Lectin