Rabbit anti-Mouse SDF-1 Polyclonal Antibody | anti-CXCL12 antibody

Anti-SDF-1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8110802

• Gene Names: Cxcl12; Pbsf; Sdf1; Tlsf; Tpar1; Scyb12
• Reactivity: Mouse
• Applications: Western Blot, ELISA
• Purity: Protein A & Antigen Affinity
• Synonyms: SDF-1; Polyclonal Antibody; Anti-SDF-1 Antibody; Rabbit Polyclonal; CXCL12/SDF-1 beta Antibody; Rabbit PAb; Antigen Affinity Purified; chemokine (C-X-C motif) ligand 12; Anti-Pbsf Antibody; Anti-Scyb12 Antibody; Anti-Sdf1 Antibody; Anti-Tlsf Antibody; Anti-Tpar1 Antibody; anti-CXCL12 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse CXCL12/SDF-1 beta
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CXCL12 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500-1:1000; ELISA: 1:25,000-1:50,000
• Immunogen: Recombinant Mouse CXCL12/SDF-1 beta Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Mouse CXCL12/SDF-1 beta (rM CXCL12/SDF-1 beta). CXCL12/SDF-1 beta specific IgG was purified by Mouse CXCL12/SDF-1 beta affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-CXCL12 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:10 kDaObserved band size:13 kDa)

Related Product Information for anti-CXCL12 antibody

The human stromal cell-derived factor-1 (SDF1), also known as CXCL12, is a small (8 kDa) cytokine highly conserved chemotactic cytokine belonging to the large family of CXC chemokines. SDF1 is expressed in two isoforms from a single gene that encodes two splice variants, SDF1alpha and SDF1beta, which are identical except for the four residues present in the C-terminus of SDF1beta but absent from SDF1alpha. The chemokine CXCL12 [stromal cell-derived factor-1 (SDF-1)] binds primarily to CXC receptor 4 (CXCR4; CD184). The binding of CXCL12 to CXCR4 induces intracellular signaling through several divergent pathways initiating signals related to chemotaxis, cell survival and/or proliferation, increase in intracellular calcium, and gene transcription. CXCL12 and CXCR4 that have been widely characterized in peripheral tissues and delineate their main functions in the CNS. Extensive evidence supports CXCL12 as a key regulator for early development of the CNS. In the mature CNS, CXCL12 modulates neurotransmission, neurotoxicity and neuroglial interactions. CXCL12 has crucial roles in the formation of multiple organ systems during embryogenesis and in the regulation of bone marrow haematopoiesis and immune function in the postnatal organism. Although considered an important factor in normal bone metabolism, recent studies implicate CXCL12 in the pathogenesis of several diseases involving the skeleton, including rheumatoid arthritis and cancers that metastasize to bone. The CXCL12/CXCR4 axis is involved in tumor progression, angiogenesis, metastasis, and survival. Pathologically enhanced CXCL12 signaling may promote the formation of new vessels through recruiting circulating endothelial progenitor cells or directly enhancing the migration/growth of endothelial cells. Therefore, CXCL12 signaling represents an important mechanism that regulates brain tumor angiogenesis/vasculogenesis and may provide potential targets for anti-angiogenic therapy in malignant gliomas.

Product Categories/Family for anti-CXCL12 antibody

Neuroinflammation

References

Bleul, C.C. et al., 1996, Nature. 382: 829-833. Sapede, D. et al., 2005, Proc. Natl. Acad. Sci. USA. 102: 1714-1718. Delgado, M.B. et al., 2001, Eur. J. Immunol. 31: 699-707. Orimo, A. et al., 2005, Cell. 121: 335-348. Kryczek, I. et al., 2007, Am. J. Physiol. Cell. Physiol. 292: C987-995. Bbalabanian, K. et al., 2005, J. Biol. Chem. 280: 35760-35766.

NCBI and Uniprot Product Information

• NCBI GI #: 7305465
• NCBI GeneID: 20315
• NCBI Accession #: NP_038683.1
• NCBI GenBank Nucleotide #: NM_013655.4
• UniProt Accession #: P40224; Q4FJL5; Q543V6
• Molecular Weight: 10,032 Da
• NCBI Official Full Name: stromal cell-derived factor 1 isoform beta
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) ligand 12
• NCBI Official Symbol: Cxcl12
• NCBI Official Synonym Symbols: Pbsf; Sdf1; Tlsf; Tpar1; Scyb12
• NCBI Protein Information: stromal cell-derived factor 1
• UniProt Protein Name: Stromal cell-derived factor 1
• Protein Family: Stromal cell-derived factor
• UniProt Gene Name: Cxcl12
• UniProt Synonym Gene Names: Sdf1; SDF-1; TPAR1; PBSF; TLSF
• UniProt Entry Name: SDF1_MOUSE

Uniprot Description

Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells (). Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation.