LDLRAP1 recombinant protein

LDLRAP1, 1-308aa, Human, His tag, E Coli

Cat. Num. AAA204342

• Gene Names: LDLRAP1; ARH; ARH1; ARH2; FHCB1; FHCB2
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: LDLRAP1; 1-308aa; Human; His tag; E Coli; Low density lipoprotein receptor adaptor protein 1; ARH; ARH1; ARH2; FHCB1; FHCB2; LDLRAP1 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 2mM DTT, 10% glycerol, 200mM NaCl
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MDALKSAGRA LIRSPSLAKQ SWGGGGRHRK LPENWTDTRE TLLEGMLFSL KYLGMTLVEQ PKGEELSAAA IKRIVATAKA SGKKLQKVTL KVSPRGIILT DNLTNQLIEN VSIYRISYCT ADKMHDKVFA YIAQSQHNQS LECHAFLCTK RKMAQAVTLT VAQAFKVAFE FWQVSKEEKE KRDKASQEGG DVLGARQDCT PPLKSLVATG NLLDLEETAK APLSTVSANT TNMDEVPRPQ ALSGSSVVWE LDDGLDEAFS RLAQSRTNPQ VLDTGLTAQD MHYAQCLSPV DWDKPDSSGT EQDDLFSF
• Sequence Length: 308

• Applicable Applications for LDLRAP1 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for LDLRAP1 recombinant protein

LDLRAP1 (Low density lipoprotein receptor adapter protein 1) is a cytosolic protein which contains a phosphotyrosine binding (PTD) domain. LDLRAP1 is a cytosolic adaptor that couples LDLR to endocytic machinery. Defects in LDLRAP1 are the cause of autosomal recessive hypercholesterolemia (ARH). ARH is a disorder caused by defective internalization of LDL receptors (LDLR) in the liver. Recombinant human LDLRAP1 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for LDLRAP1 recombinant protein

Metabolism

References

Garcia C.K., et al. (2001) Science 292:1394-1398; Mishra S.K., et al. (2005) J. Biol. Chem. 280:19270-19280

NCBI and Uniprot Product Information

• NCBI GI #: 127796563
• NCBI GeneID: 26119
• Molecular Weight: 36.1kDa (328aa), confirmed by MALDI-TOF
• NCBI Official Full Name: Low density lipoprotein receptor adaptor protein 1
• NCBI Official Synonym Full Names: low density lipoprotein receptor adaptor protein 1
• NCBI Official Symbol: LDLRAP1
• NCBI Official Synonym Symbols: ARH; ARH1; ARH2; FHCB1; FHCB2
• NCBI Protein Information: low density lipoprotein receptor adapter protein 1
• UniProt Protein Name: Low density lipoprotein receptor adapter protein 1
• Protein Family: Low density lipoprotein receptor adapter protein
• UniProt Gene Name: LDLRAP1

NCBI Description

The protein encoded by this gene is a cytosolic protein which contains a phosphotyrosine binding (PTD) domain. The PTD domain has been found to interact with the cytoplasmic tail of the LDL receptor. Mutations in this gene lead to LDL receptor malfunction and cause the disorder autosomal recessive hypercholesterolaemia. [provided by RefSeq, Jul 2008]

Uniprot Description

Adapter protein (clathrin-associated sorting protein (CLASP)) required for efficient endocytosis of the LDL receptor (LDLR) in polarized cells such as hepatocytes and lymphocytes, but not in non-polarized cells (fibroblasts). May be required for LDL binding and internalization but not for receptor clustering in coated pits. May facilitate the endocytocis of LDLR and LDLR-LDL complexes from coated pits by stabilizing the interaction between the receptor and the structural components of the pits. May also be involved in the internalization of other LDLR family members. Binds to phosphoinositides, which regulate clathrin bud assembly at the cell surface. Required for trafficking of LRP2 to the endocytic recycling compartment which is necessary for LRP2 proteolysis, releasing a tail fragment which translocates to the nucleus and mediates transcriptional repression ().