Rabbit ARH (LDLRAP1) Polyclonal Antibody | anti-LDLRAP1 antibody

ARH (LDLRAP1) Antibody (N-term)

Cat. Num. AAA9206647

• Gene Names: LDLRAP1; ARH; ARH1; ARH2; FHCB1; FHCB2
• Reactivity: Human (Predicted Reactivity: Mouse)
• Applications: Western Blot, ELISA, Immunohistochemistry
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: ARH (LDLRAP1); Polyclonal Antibody; ARH (LDLRAP1) Antibody (N-term); Low density lipoprotein receptor adapter protein 1; Autosomal recessive hypercholesterolemia protein; LDLRAP1; ARH; anti-LDLRAP1 antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Mouse)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This ARH (LDLRAP1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 11-42 amino acids from the N-terminal region of human ARH (LDLRAP1).
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: Vial Concentration: 2 (varies by lot)
• Sequence Positions: 11-42
• Sequence Length: 308

• Applicable Applications for anti-LDLRAP1 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of LDLRAP1 Antibody (N-term) in K562 cell line lysates (35ug/lane). LDLRAP1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with LDLRAP1 antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Related Product Information for anti-LDLRAP1 antibody

LDLRAP1 is a cytosolic protein which contains a phosphotyrosine binding (PTD) domain. The PTD domain has been found to interact with the cytoplasmic tail of the LDL receptor. This adapter protein is required for efficient endocytosis of the LDL receptor (LDLR) in polarized cells such as hepatocytes and lymphocytes, but not in non-polarized cells (fibroblasts). LDLRAP1 may be required for LDL binding and internalization but not for receptor clustering in coated pits. This protein may facilitate the endocytocis of LDLR and LDLR-LDL complexes from coated pits by stabilizing the interaction between the receptor and the structural components of the pits, and may also be involved in the internalization of other LDLR family members. Mutations in the LDLRAP1 gene lead to LDL receptor malfunction and cause the disorder autosomal recessive hypercholesterolaemia.

Product Categories/Family for anti-LDLRAP1 antibody

Cardiovascular; Metabolism; Signal transduction

References

Maurer,M.E., J. Cell. Sci. 119 (PT 20), 4235-4246 (2006)
Keyel,P.A., Mol. Biol. Cell 17 (10), 4300-4317 (2006)

NCBI and Uniprot Product Information

• NCBI GI #: 132626790
• NCBI GeneID: 26119
• NCBI Accession #: NP_056442.2
• NCBI GenBank Nucleotide #: NM_015627.2
• UniProt Accession #: Q5SW96; Q6TQS9; Q8N2Y0; Q9UFI9; A2BHI5
• Molecular Weight: 33885
• NCBI Official Full Name: low density lipoprotein receptor adapter protein 1
• NCBI Official Synonym Full Names: low density lipoprotein receptor adaptor protein 1
• NCBI Official Symbol: LDLRAP1
• NCBI Official Synonym Symbols: ARH; ARH1; ARH2; FHCB1; FHCB2
• NCBI Protein Information: low density lipoprotein receptor adapter protein 1
• UniProt Protein Name: Low density lipoprotein receptor adapter protein 1
• Protein Family: Low density lipoprotein receptor adapter protein
• UniProt Gene Name: LDLRAP1
• UniProt Synonym Gene Names: ARH
• UniProt Entry Name: ARH_HUMAN

NCBI Description

The protein encoded by this gene is a cytosolic protein which contains a phosphotyrosine binding (PTD) domain. The PTD domain has been found to interact with the cytoplasmic tail of the LDL receptor. Mutations in this gene lead to LDL receptor malfunction and cause the disorder autosomal recessive hypercholesterolaemia. [provided by RefSeq, Jul 2008]

Uniprot Description

LDLRAP1: Adapter protein (clathrin-associated sorting protein (CLASP)) required for efficient endocytosis of the LDL receptor (LDLR) in polarized cells such as hepatocytes and lymphocytes, but not in non-polarized cells (fibroblasts). May be required for LDL binding and internalization but not for receptor clustering in coated pits. May facilitate the endocytocis of LDLR and LDLR-LDL complexes from coated pits by stabilizing the interaction between the receptor and the structural components of the pits. May also be involved in the internalization of other LDLR family members. Binds to phosphoinositides, which regulate clathrin bud assembly at the cell surface. Defects in LDLRAP1 are the cause of autosomal recessive hypercholesterolemia (ARH). ARH is a disorder caused by defective internalization of LDL receptors (LDLR) in the liver. ARH has the clinical features of familial hypercholesterolemia (FH) homozygotes, including severely elevated plasma LDL cholesterol, tuberous and tendon xanthomata, and premature atherosclerosis. LDL receptor (LDLR) activity measured in skin fibroblasts is normal, as the LDL binding ability.

Chromosomal Location of Human Ortholog: 1p36.11

Cellular Component: recycling endosome; internal side of plasma membrane; AP-1 adaptor complex; axon; early endosome; basal plasma membrane; neurofilament; AP-2 adaptor complex; cytosol

Molecular Function: phosphatidylinositol-4,5-bisphosphate binding; protein binding; clathrin binding; receptor signaling complex scaffold activity; phosphotyrosine binding; low-density lipoprotein receptor binding; beta-amyloid binding

Biological Process: cholesterol metabolic process; receptor-mediated endocytosis; cholesterol homeostasis; positive regulation of signal transduction; amyloid precursor protein metabolic process; transport; regulation of protein binding; receptor internalization; positive regulation of receptor-mediated endocytosis

Disease: Hypercholesterolemia, Autosomal Recessive