Rat Low-density lipoprotein ELISA Kit | LDL elisa kit

Rat Low-density lipoprotein ELISA Kit

Cat. Num. AAA2887724

• Gene Names: LDLR; FH; FHC; LDLCQ2
• Reactivity: Rat
• Synonyms: Low-density lipoprotein; Rat Low-density lipoprotein ELISA Kit; LDL; LDL elisa kit

• Reactivity: Rat
• Specificity: This assay recognizes recombinant and natural rat Low-density lipoprotein. No significant cross-reactivity or interference was observed.
• Sequence Length: 76

• Assay Type: Sandwich
• Detection Range: 6.25-400 ng/mL
• Sensitivity: 3.165 ng/mL
• Intra-Assay CV: <=5.2%
• Inter-Assay CV: <=9.7%
• Recovery: 107%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LDL elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of rat low-density lipoprotein,Low-density lipoprotein concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Principle of the Assay||The microtiter plate provided in this kit has been pre-coated with an antibody specific to Low-density lipoprotein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Low-density lipoprotein and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Low-density lipoprotein, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Low-density lipoprotein in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 306411097
• NCBI GeneID: 3949
• Molecular Weight: 82,255 Da
• NCBI Official Full Name: low density lipoprotein, partial
• NCBI Official Synonym Full Names: low density lipoprotein receptor
• NCBI Official Symbol: LDLR
• NCBI Official Synonym Symbols: FH; FHC; LDLCQ2
• NCBI Protein Information: low-density lipoprotein receptor
• UniProt Protein Name: Low-density lipoprotein receptor
• Protein Family: Low-density lipoprotein receptor
• UniProt Gene Name: LDLR
• UniProt Synonym Gene Names: LDL receptor
• UniProt Entry Name: LDLR_HUMAN

NCBI Description

The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Sep 2010]

Uniprot Description

LDLR: Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. In case of HIV-1 infection, functions as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells. Defects in LDLR are the cause of familial hypercholesterolemia (FH); a common autosomal semi- dominant disease that affects about 1 in 500 individuals. The receptor defect impairs the catabolism of LDL, and the resultant elevation in plasma LDL-cholesterol promotes deposition of cholesterol in the skin (xanthelasma), tendons (xanthomas), and coronary arteries (atherosclerosis). Belongs to the LDLR family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: apical part of cell; basolateral plasma membrane; cell surface; coated pit; early endosome; endosome membrane; external side of plasma membrane; Golgi apparatus; integral to plasma membrane; late endosome; lysosome; membrane; plasma membrane; receptor complex

Molecular Function: calcium ion binding; clathrin heavy chain binding; glycoprotein binding; identical protein binding; low-density lipoprotein binding; low-density lipoprotein receptor activity; protease binding; protein binding; receptor activity; very-low-density lipoprotein receptor activity; viral receptor activity

Biological Process: cholesterol absorption; cholesterol homeostasis; cholesterol metabolic process; cholesterol transport; endocytosis; entry of virus into host cell; fat-soluble vitamin metabolic process; lipid metabolic process; lipoprotein catabolic process; lipoprotein metabolic process; phospholipid transport; phototransduction, visible light; receptor-mediated endocytosis; retinoid metabolic process; vitamin metabolic process

Disease: Hypercholesterolemia, Familial