Lecithin-cholesterol acyltransferase/LCAT Recombinant Protein | LCAT recombinant protein

Recombinant Human Lecithin-cholesterol acyltransferase/LCAT Protein (C-6His)

Cat. Num. AAA2552895

• Purity: >95% as determined by reducing SDS-PAGE.
• Synonyms: Lecithin-cholesterol acyltransferase/LCAT; Recombinant Human Lecithin-cholesterol acyltransferase/LCAT Protein (C-6His); Phosphatidylcholine-sterol acyltransferase; also named Lecithin-cholesterol acyltransferase; Phospholipid-cholesterol acyltransferase and LACT; is an extracellular cholesterol esterifying enzyme which belongs to the AB hydrolase superfamily; LCAT recombinant protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 mum filtered solution of 50mM Acetate Buffer pH-4.0.
• Sequence Positions: Phe25-Glu440
• Sequence Length: 440

• Species: Human
• Endotoxin: <1.0 EU per ug as determined by LAL test.
• Fusion Tag: C-6His
• Preparation and Storage: Lyophilized protein should be stored at < -20 degree C, though stable at room temperature for 3 weeks.; Reconstituted protein solution can be stored at 4-7 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.

Related Product Information for LCAT recombinant protein

Lipase family. The gene encoding this protein is expressed mainly in brain, liver and testes, followed by secreting into plasma and cerebral spinal fluid. The esterification of cholesterol is required for cholesterol transport. LCAT is a central enzyme in the extracellular metabolism of plasma lipoproteins. Defects in LCAT are the cause of lecithin-cholesterol acyltransferase deficiency (LCATD) and a cause of fish-eye disease (FED).

NCBI and Uniprot Product Information

• NCBI GI #: 4557892
• NCBI GeneID: 3931
• NCBI Accession #: NP_000220.1
• NCBI GenBank Nucleotide #: NM_000229.1
• UniProt Accession #: P04180; Q53XQ3
• Molecular Weight: Molecular Mass: 48.1 kDa; Actual Protein Molecular Mass: 66 kDa
• NCBI Official Full Name: phosphatidylcholine-sterol acyltransferase
• NCBI Official Synonym Full Names: lecithin-cholesterol acyltransferase
• NCBI Official Symbol: LCAT
• NCBI Protein Information: phosphatidylcholine-sterol acyltransferase
• UniProt Protein Name: Phosphatidylcholine-sterol acyltransferase
• UniProt Gene Name: LCAT

NCBI Description

This gene encodes the extracellular cholesterol esterifying enzyme, lecithin-cholesterol acyltransferase. The esterification of cholesterol is required for cholesterol transport. Mutations in this gene have been found to cause fish-eye disease as well as LCAT deficiency. [provided by RefSeq, Jul 2008]

Uniprot Description

Central enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs) (PubMed:10329423, PubMed:19065001, PubMed:26195816). The cholesterol ester is then transported back to the liver. Has a preference for plasma 16:0-18:2 or 18:O-18:2 phosphatidylcholines (PubMed:8820107). Also produced in the brain by primary astrocytes, and esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia and influences cerebral spinal fluid (CSF) APOE- and APOA1 levels. Together with APOE and the cholesterol transporter ABCA1, plays a key role in the maturation of glial-derived, nascent lipoproteins. Required for remodeling high-density lipoprotein particles into their spherical forms (PubMed:10722751).