Human lecithin-cholesterol acyltransferase ELISA Kit | LCAT elisa kit

Human Lecithin Cholesterol Acyltransferase, LCat ELISA Kit

Cat. Num. AAA703156

• Reactivity: Human
• Synonyms: lecithin-cholesterol acyltransferase; Human Lecithin Cholesterol Acyltransferase; LCat ELISA Kit; Human Lecithin Cholesterol Acyltransferase (LCAT) ELISA Kit; LCAT elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human LCAT. No significant cross-reactivity or interference between human LCAT and analogues was observed.
• Sequence Length: 440

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 3.12 ng/ml-200 ng/ml
• Sensitivity: < 0.78 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LCAT elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LCAT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LCAT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LCAT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LCAT bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 4557892
• NCBI GeneID: 3931
• NCBI Accession #: NP_000220.1
• NCBI GenBank Nucleotide #: NM_000229.1
• UniProt Accession #: P04180
• Molecular Weight: 49,578 Da
• NCBI Official Full Name: phosphatidylcholine-sterol acyltransferase
• NCBI Official Synonym Full Names: lecithin-cholesterol acyltransferase
• NCBI Official Symbol: LCAT
• NCBI Protein Information: phosphatidylcholine-sterol acyltransferase; phospholipid-cholesterol acyltransferase
• UniProt Protein Name: Phosphatidylcholine-sterol acyltransferase
• Protein Family: Lecithin-cholesterol acyltransferase
• UniProt Gene Name: LCAT

NCBI Description

This gene encodes the extracellular cholesterol esterifying enzyme, lecithin-cholesterol acyltransferase. The esterification of cholesterol is required for cholesterol transport. Mutations in this gene have been found to cause fish-eye disease as well as LCAT deficiency. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Central enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs). The cholesterol ester is then transported back to the liver. Has a preference for plasma 16:0-18:2 or 18:O-18:2 phosphatidylcholines. Also produced in the brain by primary astrocytes, and esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia and influences cerebral spinal fluid (CSF) APOE- and APOA1 levels. Together with APOE and the cholesterol transporter ABCA1, plays a key role in the maturation of glial-derived, nascent lipoproteins. Required for remodeling high-density lipoprotein particles into their spherical forms.1 PublicationManual assertion based on experiment in:Ref.15

Catalytic activity: Phosphatidylcholine + a sterol = 1-acylglycerophosphocholine + a sterol ester.

Enzyme regulation: APOA1 is the most potent activator in plasma. Also activated by APOE, APOC1 and APOA4.1 PublicationManual assertion based on experiment in:Ref.19