Lipopolysaccarid Recombinant Protein | mLBP recombinant protein

Recombinant Mouse Lipopolysaccarid Binding Protein

Cat. Num. AAA143090

• Gene Names: Lbp; Ly88; Bpifd2
• Applications: ELISA, Western Blot
• Synonyms: Lipopolysaccarid; Recombinant Mouse Lipopolysaccarid Binding Protein; LBP Mouse; Lipopolysaccaride Mouse Recombinant; Lipopolysaccharide-binding protein; LBP; Ly88; mLBP recombinant protein

• Host: Chinese Hamster Ovarian Cells (CHO)
• Form/Format: Recombinant Mouse LBP was lyophilized from a protein solution (1 mg/ml) containing phosphate-buffered saline, pH 7.2.; Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence Length: 481

• Applicable Applications for mLBP recombinant protein: ELISA (EIA), Western Blot (WB)
• Characterization: On SDS-PAGE commassie blue stained gel, the 95% purified recombinant protein shows a band at 58 kDa.
• Solubility: It is recommended to reconstitute the lyophilized LBP in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
• Preparation and Storage: Lyophilized LBP Mouse Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution LBP should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.Please prevent freeze-thaw cycles.

Related Product Information for mLBP recombinant protein

Description: LBP is produced from mouse LBP transfected CHO-cells in serum free medium. For transfection we have cloned complete mouse LBP cDNA into expression vector pPOL-DHFR. Before transfection the complete mouse LBP cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR. RmLBP was produced by his-tag by means of metal affinity purification with Talon and controlled by SDS page. Attention: His-tag at the c-terminal end of the LBP has no protease site and is not to split off. Up to 2 ug/ml LBP mediates binding of FITC-LPS (0.5 ug/ml) to CD14+CHO transfectants (2 x 106/ml).

Introduction: Lipopolysaccharides (LPS) are a type of glycolipids on the outer cell wall of Gram-negative bacteria. Lipopolysaccharide binding protein (aka LBP) is a plasma protein which facilitates the diffusion of bacterial LPS (endotoxin). LBP is involved in the acute-phase immunologic response to gram-negative bacterial infections. In cooperation with bactericidal permeability-increasing protein (BPI), LBP binds LPS and interacts with the CD14 receptor, most likely playing a role in regulating LPS-dependent monocyte responses. LBP belongs to a family of structurally and functionally related proteins, including BPI, plasma cholesteryl ester transfer protein (CETP), and phospholipid transfer protein (PLTP). The LBP gene is found on chromosome 20, directly downstream of the BPI gene. LBP catalyzes the transfer of LPS monomers from LPS aggregates to HDL particles, to phospholipid bilayers, and to a binding site on soluble CD14 (sCD14). sCD14 is capable of speeding up the transfer by receiving an LPS monomer from an LPS aggregate, and then yielding it to an HDL particle, therefore acting as a soluble "shuttle" for an insoluble lipid.

Product Categories/Family for mLBP recombinant protein

Recombinant & Natural Proteins; Recombinant Proteins; Lipopolysaccarid Binding Protein

NCBI and Uniprot Product Information

• NCBI GI #: 113865991
• NCBI GeneID: 16803
• NCBI Accession #: NP_032515.2
• NCBI GenBank Nucleotide #: NM_008489.2
• UniProt Accession #: Q61805; Q99KA0; A2AC66
• Molecular Weight: 53,055 Da
• NCBI Official Full Name: lipopolysaccharide-binding protein
• NCBI Official Synonym Full Names: lipopolysaccharide binding protein
• NCBI Official Symbol: Lbp
• NCBI Official Synonym Symbols: Ly88; Bpifd2
• NCBI Protein Information: lipopolysaccharide-binding protein
• UniProt Protein Name: Lipopolysaccharide-binding protein
• UniProt Gene Name: Lbp
• UniProt Synonym Gene Names: LBP
• UniProt Entry Name: LBP_MOUSE

Uniprot Description

LBP: Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria. The LBP/LPS complex seems to interact with the CD14 receptor. Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular space; cell surface; extracellular region

Molecular Function: lipopolysaccharide binding; lipid binding; receptor binding

Biological Process: positive regulation of tumor necrosis factor biosynthetic process; immune system process; detection of molecule of bacterial origin; positive regulation of toll-like receptor 4 signaling pathway; positive regulation of interleukin-6 production; response to lipopolysaccharide; negative regulation of tumor necrosis factor production; lipid transport; positive regulation of tumor necrosis factor production; defense response to Gram-negative bacterium; leukocyte chemotaxis during inflammatory response; positive regulation of chemokine production; positive regulation of interleukin-8 production; lipopolysaccharide transport; defense response to Gram-positive bacterium; transport; macrophage activation during immune response; defense response to bacterium; lipopolysaccharide-mediated signaling pathway; acute-phase response; innate immune response; positive regulation of phagocytosis, engulfment; positive regulation of macrophage activation