Mouse lipopolysaccharide binding protein ELISA Kit | LBP elisa kit

Mouse Lipopolysaccharide-binding protein, LBP ELISA Kit

Cat. Num. AAA947489

• Gene Names: Lbp; Ly88; Bpifd2
• Reactivity: Mouse
• Synonyms: lipopolysaccharide binding protein; Mouse Lipopolysaccharide-binding protein; LBP ELISA Kit; Mouse Lipopolysaccharide-binding protein (LBP) ELISA kit; MGC22233; LPS-binding protein; lipopolysaccharide-binding protein; LBP elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse LBP. No significant cross-reactivity or interference between mouse LBP and analogues was observed.
• Sequence Length: 481

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 12.5 ng/ml-800 ng/ml
• Sensitivity: < 3.12 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LBP elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LBP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LBP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LBP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LBP bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 113865991
• NCBI GeneID: 16803
• NCBI Accession #: NP_032515.2
• NCBI GenBank Nucleotide #: NM_008489.2
• UniProt Accession #: Q61805; Q99KA0; A2AC66
• Molecular Weight: 53,055 Da
• NCBI Official Full Name: lipopolysaccharide-binding protein
• NCBI Official Synonym Full Names: lipopolysaccharide binding protein
• NCBI Official Symbol: Lbp
• NCBI Official Synonym Symbols: Ly88; Bpifd2
• NCBI Protein Information: lipopolysaccharide-binding protein
• UniProt Protein Name: Lipopolysaccharide-binding protein
• Protein Family: Lipopolysaccharide-binding protein
• UniProt Gene Name: Lbp
• UniProt Synonym Gene Names: LBP
• UniProt Entry Name: LBP_MOUSE

Uniprot Description

LBP: Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria. The LBP/LPS complex seems to interact with the CD14 receptor. Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: extracellular space; cell surface; extracellular region

Molecular Function: lipopolysaccharide binding; lipid binding; receptor binding

Biological Process: positive regulation of tumor necrosis factor biosynthetic process; immune system process; detection of molecule of bacterial origin; positive regulation of toll-like receptor 4 signaling pathway; positive regulation of interleukin-6 production; response to lipopolysaccharide; negative regulation of tumor necrosis factor production; defense response to Gram-negative bacterium; lipid transport; positive regulation of tumor necrosis factor production; leukocyte chemotaxis during inflammatory response; positive regulation of chemokine production; positive regulation of interleukin-8 production; lipopolysaccharide transport; defense response to Gram-positive bacterium; transport; defense response to bacterium; macrophage activation during immune response; lipopolysaccharide-mediated signaling pathway; acute-phase response; innate immune response; positive regulation of phagocytosis, engulfment; positive regulation of macrophage activation