Human Caspase-1 ELISA Kit | CASP1 elisa kit

Human Caspase-1 ELISA Kit

Cat. Num. AAA765692

• Gene Names: Casp1; Ice; Il1bc
• Reactivity: Human
• Synonyms: Caspase-1; Human Caspase-1 ELISA Kit; CASP1 (Caspase 1)/Caspase-1/ICE/CASP-1/ICEP45/IL-1 beta-converting enzyme/IL-1BC/IL1BCCASP1 nirs variant 1/IL1BCE/IL1B-convertase/interleukin 1-B converting enzyme/interleukin 1-beta convertase; CASP1 elisa kit

• Reactivity: Human
• Sequence Length: 402

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 78.125-5000pg/ml
• Sensitivity: 46.875pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for CASP1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human CASP1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human CASP1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human CASP1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human CASP1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 31542341
• NCBI GeneID: 25166
• NCBI Accession #: NP_036894.2
• NCBI GenBank Nucleotide #: NM_012762.2
• Molecular Weight: 45,576 Da
• NCBI Official Full Name: caspase-1
• NCBI Official Synonym Full Names: caspase 1
• NCBI Official Symbol: Casp1
• NCBI Official Synonym Symbols: Ice; Il1bc
• NCBI Protein Information: caspase-1
• UniProt Protein Name: Caspase-1
• Protein Family: Caspase
• UniProt Gene Name: Casp1
• UniProt Synonym Gene Names: Il1bc; Il1bce; CASP-1; IL-1BC; ICE; IL-1 beta-converting enzyme
• UniProt Entry Name: CASP1_RAT

NCBI Description

apoptotic protease that may play a role in inflammation and apoptosis in male sex organs [RGD, Feb 2006]

Uniprot Description

CASP1: Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis (By similarity)

Protein type: EC 3.4.22.36; Protease; Apoptosis