LAIR1 recombinant protein

Recombinant Mouse LAIR1 Protein (C-6His)

Cat. Num. AAA2553569

• Gene Names: Lair1; Lair-1; BB115266; D7Bwg0421e; 5133400O11Rik
• Purity: >90% as determined by reducing SDS-PAGE.
• Synonyms: LAIR1; Recombinant Mouse LAIR1 Protein (C-6His); Leukocyte-associated immunoglobulin-like receptor 1; LAIR-1; mLAIR-1; CD305; Lair1; LAIR1 recombinant protein

• Host: Human Cells
• Purity/Purification: >90% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 mum filtered solution of PBS, pH 7.4.
• Sequence Positions: Gln22-Tyr141
• Sequence Length: 263

• Species: Mouse
• Endotoxin: <1.0 EU per mug as determined by LAL test.
• Fusion Tag: C-6His
• Preparation and Storage: Lyophilized protein should be stored at < -20 degree C, though stable at room temperature for 3 weeks.; Reconstituted protein solution can be stored at 4-7 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.

Related Product Information for LAIR1 recombinant protein

Leukocyte-associated Ig-like receptor-1 (LAIR-1) is an inhibitory receptor of the Ig superfamily that is structurally related to inhibitory members of KIR and ILT/CD85 families. It is expressed on immune cells, including NK cells, T cells, B cells, monocytes, immature neutrophils, dendritic cells and most thymocytes.The 253 amino acid (aa) type I transmembrane (TM) protein contains a 21 aa signal sequence, a 124 aa extracellular domain (ECD), a 20 aa TM domain and a 98 aa cytoplasmic domain. The ECD includes one C2-type Ig-like domain and two potential N-linked glycosylation sites. Tyrosine phosphorylation of two cytoplasmic ITIM motifs results in recruitment of phosphatases and down-regulation of signaling through activating receptors. LAIR1 shows high-affinity binding of collagens that results in inhibition of degranulation in a basophilic leukemia cell line.

NCBI and Uniprot Product Information

• NCBI GI #: 164698422
• NCBI GeneID: 52855
• NCBI Accession #: NP_001106945.1
• NCBI GenBank Nucleotide #: NM_001113474.1
• UniProt Accession #: Q8BG84; Q80UN5; Q8BHB6; Q8BRR6; Q8C6N8; Q8CEP5
• Molecular Weight: Molecular Mass: 14.4 kDa; Actual Protein Molecular Mass: 15-29kDa
• NCBI Official Full Name: leukocyte-associated immunoglobulin-like receptor 1 isoform a
• NCBI Official Synonym Full Names: leukocyte-associated Ig-like receptor 1
• NCBI Official Symbol: Lair1
• NCBI Official Synonym Symbols: Lair-1; BB115266; D7Bwg0421e; 5133400O11Rik
• NCBI Protein Information: leukocyte-associated immunoglobulin-like receptor 1
• UniProt Protein Name: Leukocyte-associated immunoglobulin-like receptor 1
• Protein Family: Leukocyte-associated immunoglobulin-like receptor
• UniProt Gene Name: Lair1
• UniProt Synonym Gene Names: LAIR-1; mLAIR1

Uniprot Description

Functions as an inhibitory receptor that plays a constitutive negative regulatory role on cytolytic function of natural killer (NK) cells, B-cells and T-cells. Activation by Tyr phosphorylation results in recruitment and activation of the phosphatases PTPN6 and PTPN11. It also reduces the increase of intracellular calcium evoked by B-cell receptor ligation. May also play its inhibitory role independently of SH2-containing phosphatases. Modulates cytokine production in CD4+ T-cells, down-regulating IL2 and IFNG production while inducing secretion of transforming growth factor beta. Down-regulates also IgG and IgE production in B-cells as well as IL8, IL10 and TNF secretion. Inhibits proliferation and induces apoptosis in myeloid leukemia cell lines as well as prevents nuclear translocation of NF-kappa-B p65 subunit/RELA and phosphorylation of I-kappa-B alpha/CHUK in these cells. Inhibits the differentiation of peripheral blood precursors towards dendritic cells ().