Testing Data
KSR1 recombinant protein
KSR1 (L639F), Active
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Testing Data
Testing Data
Testing Data #2
Testing Data #2
Scientific Background: KSR1 or Kinase Suppressor of Ras 1 interacts with various kinases of the Raf/MEK/extracellular signal-regulated kinase pathway to enhance its activation. KSR1 is regulated in response to a specific mode of dimerization of its kinase domain, which is termed the side-to-side dimer, whereas KSR1 also participates in forming side-to-side heterodimers with RAF and this can trigger RAF activation. KSR1 has an essential protective role in the intestinal epithelial cell during inflammation through activation of cell survival pathways (1). KSR1 functions as a scaffold that enhances iNOS activity and is crucial for the pulmonary response to P. aeruginosa infection (2).
NCBI and Uniprot Product Information
Uniprot Description
KSR: a serine/threonine kinase of the Raf family with no demonstrated kinase activity. A location-regulated scaffolding protein connecting MEK to RAF. Promotes MEK and RAF phosphorylation and activity through assembly of an activated signaling complex. Interacts with RAF and MAPK/ERK, in a Ras-dependent manner. The binding of 14-3-3 proteins to phosphorylated KSR prevents the membrane localization. In unstimulated cells, the phosphorylated form is bound to a 14-3-3 protein and sequestered in the cytoplasm. Translocates from the cytoplasm to the cell periphery following TNF treatment. When Bad is available, ceramide signaling through the Ras/Raf cascade can lead to apoptosis. Three alternatively-spliced isoforms have been described.
Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; RAF family; TKL group
Chromosomal Location of Human Ortholog: 17q11.2
Cellular Component: cytosol; endoplasmic reticulum; membrane; protein complex
Molecular Function: MAP-kinase scaffold activity; protein binding; protein C-terminus binding; protein kinase activity; signal transducer activity
Biological Process: cAMP-mediated signaling; MAPKKK cascade; positive regulation of MAPKKK cascade; protein amino acid phosphorylation; Ras protein signal transduction
Research Articles on KSR1
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Product Notes
The KSR1 ksr1 (Catalog #AAA515515) is a Recombinant Protein produced from Sf9 insect cells using baculovirus and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 335-end (L639F). AAA Biotech's KSR1 can be used in a range of immunoassay formats including, but not limited to, Kinase Assay. Researchers should empirically determine the suitability of the KSR1 ksr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KSR1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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